RT-PCR for EBV Transcripts in BL Tumors and Cell Lines
| Samples . | BART . | EBNA1 . | EBNA2 . | LMP1 . | LMP2 . | BZLF1 . | BHRF1 . | BLLF1 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FUK . | QUK . | YUK . | 2A . | 2B . | Latent . | Lytic . | |||||||
| BL cell lines | Rael | ++ | + | ++ | − | − | − | ++ | − | +/− | + | ++ | ++ |
| Akata | ++ | ++ | ++ | − | − | − | ++ | − | + | − | ++ | ++ | |
| Chep | ++ | ++ | ++ | − | − | − | ++ | − | ++ | − | ++ | ++ | |
| Wan | ++ | ++ | ++ | ++ | + | ++ | ++ | − | ++ | +/− | ++ | ++ | |
| Wewak | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | |
| Namalwa | ++ | ++ | ++* | ++ | ++ | ++ | − | − | − | ++ | +/− | − | |
| Rael-AzaC | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | |
| Raji | ++ | ++ | ++* | ++ | ++ | ++ | +/− | ++ | +/− | ++ | ++ | + | |
| AG876 | ++ | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ | ND | ++ | ||
| LCL | IB4 | ++ | ++ | ++* | ++ | ++ | ++ | ++ | ++ | +/− | ++ | ++ | ++ |
| B95-8 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ | |
| eBL | BL1 | ++ | − | ++ | − | − | − | + | − | − | +/− | +/− | +/− |
| BL2 | ++ | − | + | − | − | − | − | − | − | − | − | − | |
| BL6 | ++ | − | ++ | − | − | − | + | − | − | − | +/− | +/− | |
| BL7 | ++ | − | ++ | − | − | − | + | − | − | − | +/− | − | |
| BL8 | ++ | − | ++ | − | − | − | +/− | − | − | − | − | − | |
| BL9 | ++ | − | ++* | − | − | − | − | − | − | − | − | − | |
| BL10 | ++ | − | ++ | +/− | +/− | − | + | − | − | +/− | +/− | +/− | |
| NPC | NPC1 | ++ | +/− | ++ | − | − | − | + | − | − | − | − | − |
| NPC2 | ++ | +/− | ++ | − | − | − | ++ | − | − | − | +/− | − | |
| PTLD | PTLD1 | ++ | +/− | ++ | − | − | + | ++ | − | ++ | + | ++ | ++ |
| PTLD2 | ++ | +/− | ++ | − | − | − | − | − | ++ | +/− | ++ | + | |
| Samples . | BART . | EBNA1 . | EBNA2 . | LMP1 . | LMP2 . | BZLF1 . | BHRF1 . | BLLF1 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FUK . | QUK . | YUK . | 2A . | 2B . | Latent . | Lytic . | |||||||
| BL cell lines | Rael | ++ | + | ++ | − | − | − | ++ | − | +/− | + | ++ | ++ |
| Akata | ++ | ++ | ++ | − | − | − | ++ | − | + | − | ++ | ++ | |
| Chep | ++ | ++ | ++ | − | − | − | ++ | − | ++ | − | ++ | ++ | |
| Wan | ++ | ++ | ++ | ++ | + | ++ | ++ | − | ++ | +/− | ++ | ++ | |
| Wewak | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | |
| Namalwa | ++ | ++ | ++* | ++ | ++ | ++ | − | − | − | ++ | +/− | − | |
| Rael-AzaC | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | |
| Raji | ++ | ++ | ++* | ++ | ++ | ++ | +/− | ++ | +/− | ++ | ++ | + | |
| AG876 | ++ | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ | ND | ++ | ||
| LCL | IB4 | ++ | ++ | ++* | ++ | ++ | ++ | ++ | ++ | +/− | ++ | ++ | ++ |
| B95-8 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ | |
| eBL | BL1 | ++ | − | ++ | − | − | − | + | − | − | +/− | +/− | +/− |
| BL2 | ++ | − | + | − | − | − | − | − | − | − | − | − | |
| BL6 | ++ | − | ++ | − | − | − | + | − | − | − | +/− | +/− | |
| BL7 | ++ | − | ++ | − | − | − | + | − | − | − | +/− | − | |
| BL8 | ++ | − | ++ | − | − | − | +/− | − | − | − | − | − | |
| BL9 | ++ | − | ++* | − | − | − | − | − | − | − | − | − | |
| BL10 | ++ | − | ++ | +/− | +/− | − | + | − | − | +/− | +/− | +/− | |
| NPC | NPC1 | ++ | +/− | ++ | − | − | − | + | − | − | − | − | − |
| NPC2 | ++ | +/− | ++ | − | − | − | ++ | − | − | − | +/− | − | |
| PTLD | PTLD1 | ++ | +/− | ++ | − | − | + | ++ | − | ++ | + | ++ | ++ |
| PTLD2 | ++ | +/− | ++ | − | − | − | − | − | ++ | +/− | ++ | + | |
RT-PCR signal judged by autoradiography was indicated as ++, +, and +/−, as described in Materials and Methods.
Abbreviation: ND, not done.
Weakly positive or negative for QUK, but positive for Q'UK.