Oligonucleotides Used in RT-PCR for EBV Transcripts
| Transcripts . | Oligos . | Oligonucleotide Sequence . | Coordinate in B95-8 . | Annealing Temperature . | Reference . |
|---|---|---|---|---|---|
| BART | A3 | 5′-AGAGACCAGGCTGCTAAACA | 157154-157173 | 55°C | 16 |
| A4 | 5-AACCAGCTTTCCTTTCCGAG | 159194-159175 | |||
| Probe | 5′-AAGACGTTGGAGGCACGCTG | 157359-157378 | |||
| EBNA1 | F | 5'-GGATCCGGAGGGGACCACTA | 62249-62268 | 55°C | 15 |
| Q′ | 5'-GCGGGATAGCGTGCGCTA | 62430-62447 | 5 | ||
| Q | 5′-GTGCGCTACCGGATGGCG | 62440-62457 | |||
| Y3 | 5′-TGGCGTGTGACGTGGTGTAA | 48397-48416 | |||
| K | 5′-CATTTCCAGGTCCTGTACCT | 107986-107967 | |||
| Probe | 5′-ATGCCCTGAGACTACTCTCT | 67563-67544 | |||
| EBNA2 | Y2 | 5′-ATTAGAGACCACTTTGAGCC | 47902-47921 | 58°C | |
| Y/P | 5′-CCCCATGTAACGCAAGATAG | 48534-48515 | |||
| Probe (Y3) | 5′-TGGCGTGTGACGTGGTGTAA | 48397-48416 | |||
| LMP1 | Primer | 5′-GTGACTGGACTGGAGGAGCC | 169341-169322 | 60°C | |
| Primer | 5′-GAGGGAGTCATCGTGGTGGTG | 168718-168738 | |||
| Probe | 5′-AGCCCTCCTTGTCCTCTA | 169325-169308 | |||
| LMP2A | T1 | 5′-GCAACACGACGGGAATGACG | 166824-166843 | 58°C | 33 |
| T1′ | 5′-TGGGAAGCGGCAGTGTAATC | 169809-169828 | |||
| T2 | 5′-AAACACGAGGCGGCAATAGC | 131-112 | |||
| Probe | 5′-ATCCAGTATGCCTGCCTGTA | 62-81 | |||
| BZLF1 | Primer | 5′-GGGAGAAGCACCTCAACCTG | 102826-102807 | 58°C | |
| Primer | 5′-TTGCTTAAACTTGGCCCGGC | 102447-102466 | |||
| Probe | 5′-AGCCAGAATC/CTGGAGGAAT | 102665-102656/ | |||
| 102530-102521 | |||||
| BHRF1 | Y2 (latent) | 5′-ATTAGAGACCACTTTGAGCC | 47902-47921 | 55°C | 50 |
| H2 (lytic) | 5′-GTCAAGGTTTCGTCTGTGTG | 53830-53849 | |||
| H3 | 5′-TTCTCTTGCTGCTAGCTCCA | 54461-54480 | |||
| Probe | 5′-CTGTCCCGTATACACAGGGC | 54425-54406 | |||
| BLLF1 | Primer | 5′-GTGGATGTGGAACTGTTTCCAG | 89934-89955 | 63°C | |
| (gp220) | Primer | 5′-CTGTATCCACCGCGGATGTCAC | 90753-90732 | ||
| Probe | 5′-AGTCCATCTCCATGGGACAA | 90682-90663 |
| Transcripts . | Oligos . | Oligonucleotide Sequence . | Coordinate in B95-8 . | Annealing Temperature . | Reference . |
|---|---|---|---|---|---|
| BART | A3 | 5′-AGAGACCAGGCTGCTAAACA | 157154-157173 | 55°C | 16 |
| A4 | 5-AACCAGCTTTCCTTTCCGAG | 159194-159175 | |||
| Probe | 5′-AAGACGTTGGAGGCACGCTG | 157359-157378 | |||
| EBNA1 | F | 5'-GGATCCGGAGGGGACCACTA | 62249-62268 | 55°C | 15 |
| Q′ | 5'-GCGGGATAGCGTGCGCTA | 62430-62447 | 5 | ||
| Q | 5′-GTGCGCTACCGGATGGCG | 62440-62457 | |||
| Y3 | 5′-TGGCGTGTGACGTGGTGTAA | 48397-48416 | |||
| K | 5′-CATTTCCAGGTCCTGTACCT | 107986-107967 | |||
| Probe | 5′-ATGCCCTGAGACTACTCTCT | 67563-67544 | |||
| EBNA2 | Y2 | 5′-ATTAGAGACCACTTTGAGCC | 47902-47921 | 58°C | |
| Y/P | 5′-CCCCATGTAACGCAAGATAG | 48534-48515 | |||
| Probe (Y3) | 5′-TGGCGTGTGACGTGGTGTAA | 48397-48416 | |||
| LMP1 | Primer | 5′-GTGACTGGACTGGAGGAGCC | 169341-169322 | 60°C | |
| Primer | 5′-GAGGGAGTCATCGTGGTGGTG | 168718-168738 | |||
| Probe | 5′-AGCCCTCCTTGTCCTCTA | 169325-169308 | |||
| LMP2A | T1 | 5′-GCAACACGACGGGAATGACG | 166824-166843 | 58°C | 33 |
| T1′ | 5′-TGGGAAGCGGCAGTGTAATC | 169809-169828 | |||
| T2 | 5′-AAACACGAGGCGGCAATAGC | 131-112 | |||
| Probe | 5′-ATCCAGTATGCCTGCCTGTA | 62-81 | |||
| BZLF1 | Primer | 5′-GGGAGAAGCACCTCAACCTG | 102826-102807 | 58°C | |
| Primer | 5′-TTGCTTAAACTTGGCCCGGC | 102447-102466 | |||
| Probe | 5′-AGCCAGAATC/CTGGAGGAAT | 102665-102656/ | |||
| 102530-102521 | |||||
| BHRF1 | Y2 (latent) | 5′-ATTAGAGACCACTTTGAGCC | 47902-47921 | 55°C | 50 |
| H2 (lytic) | 5′-GTCAAGGTTTCGTCTGTGTG | 53830-53849 | |||
| H3 | 5′-TTCTCTTGCTGCTAGCTCCA | 54461-54480 | |||
| Probe | 5′-CTGTCCCGTATACACAGGGC | 54425-54406 | |||
| BLLF1 | Primer | 5′-GTGGATGTGGAACTGTTTCCAG | 89934-89955 | 63°C | |
| (gp220) | Primer | 5′-CTGTATCCACCGCGGATGTCAC | 90753-90732 | ||
| Probe | 5′-AGTCCATCTCCATGGGACAA | 90682-90663 |