Geometry and Related Parameters of RBC Before and After HU Treatment
| . | MPD . | MPD + HU . | Controls . |
|---|---|---|---|
| Diameter (μm) | 8.12 ± 0.12 | 8.92 ± 0.073 | 8.10 ± 0.09 |
| Thoroid (μm) | 2.58 ± 0.03 | 2.97 ± 0.064 | 2.60 ± 0.04 |
| Central (μm) | 1.10 ± 0.08 | 1.12 ± 0.10 | 1.11 ± 0.04 |
| Area (μm2) | 141.3 ± 3.5 | 174.7 ± 3.64 | 141.4 ± 3.0 |
| Volume (μm3) | 104.9 ± 2.6 | 145.3 ± 4.44 | 105.7 ± 3.3 |
| Area/Vol (—) | 1.368 ± 0.015 | 1.224 ± 0.0155 | 1.359 ± 0.016 |
| Surface area index (—) | 1.320 ± 0.015 | 1.314 ± 0.008 | 1.314 ± 0.006 |
| MCD (μm) | 3.20 ± 0.04 | 3.57 ± 0.055 | 3.22 ± 0.04 |
| MCD >3.5 μm (%) | 16.8 ± 2.9 | 58.8 ± 6.94 | 17.8 ± 3.7 |
| CMSM (μm3) | 0.125 ± 0.003 | 0.154 ± 0.0045 | 0.126 ± 0.03 |
| CMBT (nN/μm2) | 501 ± 26 | 532 ± 9 | 490 ± 11 |
| . | MPD . | MPD + HU . | Controls . |
|---|---|---|---|
| Diameter (μm) | 8.12 ± 0.12 | 8.92 ± 0.073 | 8.10 ± 0.09 |
| Thoroid (μm) | 2.58 ± 0.03 | 2.97 ± 0.064 | 2.60 ± 0.04 |
| Central (μm) | 1.10 ± 0.08 | 1.12 ± 0.10 | 1.11 ± 0.04 |
| Area (μm2) | 141.3 ± 3.5 | 174.7 ± 3.64 | 141.4 ± 3.0 |
| Volume (μm3) | 104.9 ± 2.6 | 145.3 ± 4.44 | 105.7 ± 3.3 |
| Area/Vol (—) | 1.368 ± 0.015 | 1.224 ± 0.0155 | 1.359 ± 0.016 |
| Surface area index (—) | 1.320 ± 0.015 | 1.314 ± 0.008 | 1.314 ± 0.006 |
| MCD (μm) | 3.20 ± 0.04 | 3.57 ± 0.055 | 3.22 ± 0.04 |
| MCD >3.5 μm (%) | 16.8 ± 2.9 | 58.8 ± 6.94 | 17.8 ± 3.7 |
| CMSM (μm3) | 0.125 ± 0.003 | 0.154 ± 0.0045 | 0.126 ± 0.03 |
| CMBT (nN/μm2) | 501 ± 26 | 532 ± 9 | 490 ± 11 |
Patients with newly diagnosed MPD were analyzed before and after treatment with HU (n = 5). Healthy controls were studied in parallel (n = 5 + 5). A drop of whole blood was suspended at low hematocrit in balanced KRH medium and introduced into a chamber under the microscope. RBCs hanging vertically from the upper chamber cover slip were focused on and, from interactive measurements of the RBC diameter, thoroidal thickness, and center thickness, the cell geometry and deformability parameters were calculated. This geometry was based on a computer iterative search for the RBC curvature polynoma equation (see equation 1). The membrane area, cell volume, area-to-volume ratio, and surface area index are given. The MCD was computerized representing the minimum pore diameter the RBC can pass through without lysis. The RBC corpuscle membrane section modulus (CMSM) and the corpuscle membrane bending tension (CMBT) were also generated from a static RBC bending model. Five levels of significances were tested: P < .051, P < .022, P < .013, P < .0054, and P < .0015. No significant differences were found between patients with MPD before treatment and controls.