Morphology of Myeloid Colonies From 4 Patient Samples
| Patient No. . | Colony Type (%) . | No. of Colonies Examined . | ||||||
|---|---|---|---|---|---|---|---|---|
| GM . | Eo . | GM/Eo . | ||||||
| LNL6 . | SCL . | LNL6 . | SCL . | LNL6 . | SCL . | LNL6 . | SCL . | |
| 1 | 50 | 52 | 35 | 26 | 15 | 22 | 48 | 66 |
| 2 | 60 | 66 | 23 | 17 | 17 | 17 | 187 | 172 |
| 3 | 70 | 53 | 24 | 36 | 6 | 11 | 163 | 237 |
| 4 | 56 | 58 | 32 | 35 | 12 | 7 | 65 | 85 |
| Mean | 59 ± 8 | 57 ± 6 | 29 ± 6 | 29 ± 9 | 12 ± 5 | 14 ± 7 | ||
| Patient No. . | Colony Type (%) . | No. of Colonies Examined . | ||||||
|---|---|---|---|---|---|---|---|---|
| GM . | Eo . | GM/Eo . | ||||||
| LNL6 . | SCL . | LNL6 . | SCL . | LNL6 . | SCL . | LNL6 . | SCL . | |
| 1 | 50 | 52 | 35 | 26 | 15 | 22 | 48 | 66 |
| 2 | 60 | 66 | 23 | 17 | 17 | 17 | 187 | 172 |
| 3 | 70 | 53 | 24 | 36 | 6 | 11 | 163 | 237 |
| 4 | 56 | 58 | 32 | 35 | 12 | 7 | 65 | 85 |
| Mean | 59 ± 8 | 57 ± 6 | 29 ± 6 | 29 ± 9 | 12 ± 5 | 14 ± 7 | ||
BM or PBPC CD34+ cells were transduced with either LNL6 or LNC(SCL) and placed into agar culture in the presence of purified, recombinant human GM-CSF (100 ng/mL), G-CSF (500 U/mL), and SCF (100 ng/mL). Agar cultures were fixed and then stained with Luxol-Fast blue. Colonies were examined under light microscope (×100) and characterized as granulocyte/macrophage (GM), eosinophil (Eo), or a mixture of both cell types (GM/Eo).