Table 1. Growth of Progenitor Cells in... | American Society of Hematology

Table 1.

Growth of Progenitor Cells in Methylcellulose After Retroviral Transduction of CD34⁺ Cells Isolated From Human Cord Blood

Experiment No.	Cell Source	Percentage of G418R Progenitors										BFU-E Ratio (S/N)	No. of Cells per BFU-E (ratio S/N)	% DAF⁺ Colonies^-150
		BFU-E		GM		G		M		Mix				% DAF⁺ Colonies^-150
		N	S	N	S	N	S	N	S	N	S			N	S
1	CB1	34	62	2.3	1.6	33	20	30	16	1.0	0	1.8	2.0	ND	ND
	CB2	44	59	1.7	1.0	22	18	33	22	0	0	1.3	1.8	ND	ND
2	CB	62	65	1.0	1.0	22	26	14	8.3	0.5	0	1.1	1.3	23	40

Experiment No.	Cell Source	Percentage of G418R Progenitors										BFU-E Ratio (S/N)	No. of Cells per BFU-E (ratio S/N)	% DAF⁺ Colonies^-150
		BFU-E		GM		G		M		Mix				% DAF⁺ Colonies^-150
		N	S	N	S	N	S	N	S	N	S			N	S
1	CB1	34	62	2.3	1.6	33	20	30	16	1.0	0	1.8	2.0	ND	ND
	CB2	44	59	1.7	1.0	22	18	33	22	0	0	1.3	1.8	ND	ND
2	CB	62	65	1.0	1.0	22	26	14	8.3	0.5	0	1.1	1.3	23	40

Retroviral transduction efficiency as determined by G418R was equivalent within each experiment. Three experiments were established using CB. Results are presented as the percentage of G418R colonies established from different progenitor cells (viz, BFU-E, GM-CFC, G-CFC, M-CFC, and Mix-CFC) after transduction with LNL6 (N) or LNC(SCL) (S). A minimum of 40 colonies were scored to obtain the various percentages.

F0-150

DAF staining was performed after culture in methylcellulose for 7 days.

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