Detection of HCV Positive and Negative Strand RNA in Different Subsets of Peripheral Hematopoietic Cells
| Cells* . | Phenotype† . | RT-PCR Strand Specificity . | Cell Range‡ (×106 cells) . | |
|---|---|---|---|---|
| Positive . | Negative . | |||
| Total PBMC | x | 11/11 (100%) | 3/11 (27%) | 1.30 to 15.00 |
| Granulocytes | CD15+ | 8/9 (89%) | 3/10 (30%) | 0.03 to 12.50 |
| Monocytes/Macrophages | CD14+ | 5/9 (56%) | 1/10 (10%) | 0.03 to 4.00 |
| B lymphocytes | CD19+ | 5/8 (63%) | 2/9 (22%) | 0.06 to 1.80 |
| T lymphocytes | CD3+ | 0/7 (0%) | 0/7 (0%) | 0.34 to 4.40 |
| Thrombocytes | negative fraction1-153 | 0/6 (0%) | 0/6 (0%) | x |
| Cells* . | Phenotype† . | RT-PCR Strand Specificity . | Cell Range‡ (×106 cells) . | |
|---|---|---|---|---|
| Positive . | Negative . | |||
| Total PBMC | x | 11/11 (100%) | 3/11 (27%) | 1.30 to 15.00 |
| Granulocytes | CD15+ | 8/9 (89%) | 3/10 (30%) | 0.03 to 12.50 |
| Monocytes/Macrophages | CD14+ | 5/9 (56%) | 1/10 (10%) | 0.03 to 4.00 |
| B lymphocytes | CD19+ | 5/8 (63%) | 2/9 (22%) | 0.06 to 1.80 |
| T lymphocytes | CD3+ | 0/7 (0%) | 0/7 (0%) | 0.34 to 4.40 |
| Thrombocytes | negative fraction1-153 | 0/6 (0%) | 0/6 (0%) | x |
*Cellular subsets are deduced according to their phenotype.
Monoclonal antibodies directed against those surface molecules were used to purify corresponding cell type as described in Materials and Methods.
Range of cells used in the PCR assays.
Negative fraction collected at the end of the magnetic selection and corresponding to the CD15, CD14, CD19, and CD3− cells. This fraction was depleted in nucleated cells and contained principally thrombocytes.