Secondary Colony Formation in the Course of BMLTC Initiated With Purified CD34+ Cells Exposed to HIV-1 and HIV-2
| Time of BMLTC . | CD34+CD38+ Cells . | CD34+CD38− Cells . | ||||
|---|---|---|---|---|---|---|
| 1 wk . | 3 wk . | LTCIC . | 1 wk . | 3 wk . | LTCIC . | |
| gp120 | 96 | 106 | 97 | 104 | 98 | 106 |
| HIV-1 Bal | 94 | 102 | 102 | 103 | 105 | 100 |
| HIV-1 Bal mock (H*) | 102 | 98 | 108 | 109 | 101 | 97 |
| HIV-1 Bal mock (R*) | 98 | 100 | 102 | 106 | 100 | 104 |
| HIV-1 RF | 97 | 98 | 107 | 110 | 102 | 98 |
| HIV-2 Rod | 105 | 96 | 110 | 97 | 102 | 108 |
| HIV-2 Rod mock (R*) | 101 | 98 | 96 | 113 | 94 | 95 |
| Sup (LPS) | 106 | 98 | 109 | 99 | 106 | 98 |
| Time of BMLTC . | CD34+CD38+ Cells . | CD34+CD38− Cells . | ||||
|---|---|---|---|---|---|---|
| 1 wk . | 3 wk . | LTCIC . | 1 wk . | 3 wk . | LTCIC . | |
| gp120 | 96 | 106 | 97 | 104 | 98 | 106 |
| HIV-1 Bal | 94 | 102 | 102 | 103 | 105 | 100 |
| HIV-1 Bal mock (H*) | 102 | 98 | 108 | 109 | 101 | 97 |
| HIV-1 Bal mock (R*) | 98 | 100 | 102 | 106 | 100 | 104 |
| HIV-1 RF | 97 | 98 | 107 | 110 | 102 | 98 |
| HIV-2 Rod | 105 | 96 | 110 | 97 | 102 | 108 |
| HIV-2 Rod mock (R*) | 101 | 98 | 96 | 113 | 94 | 95 |
| Sup (LPS) | 106 | 98 | 109 | 99 | 106 | 98 |
CFC are expressed as percentage of the mean values of control cultures for each time point (SD < 9%). All BMLTC were performed in duplicate and each culture was assessed in triplicate methylcellulose cultures to determine the number of CFC. The number of colonies in controls was 2,980 ± 270, 1,040 ± 110, 158 ± 18, 50 ± 6, and 24 ± 5 for weeks 1, 2, 3, 4, and 5, per 105CD34+ cells, respectively. The control colony numbers for CD34+CD38− sorted cells were 820 ± 58, 510 ± 58, 234 ± 18, 148 ± 11, 92 ± 10 for 1 to 5 weeks per 104 cells. Heat inactivated (H*) and irradiated virus stocks (R*) were used as indicated.