Neutrophils But Not Monocytes/Macrophages Are Present in Clonogenic Colonies From the Liver of Neonatal PU.1 Null Mice
| Experiment No. . | Cells From . | Percentage of Colonies Containing Indicated Myeloid Cell Lineages* . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MAC . | NEU . | MAST . | MAC NEU . | NEU MAST . | MAC MAST . | NEU MAC MAST . | NEU MAST MK . | NEU MAC MAST MK . | ||
| 1 | PU.1 null mice | 0 | 41 | 9 | 0 | 27 | 0 | 0 | 23 | 0 |
| Control mice | 4 | 0 | 29 | 8 | 8 | 8 | 13 | 0 | 30 | |
| 3 | PU.1 null mice | 0 | 42 | 0 | 0 | 29 | 0 | 0 | 29 | 0 |
| Control mice | 0 | 4 | 0 | 62 | 8 | 0 | 0 | 0 | 26 | |
| Experiment No. . | Cells From . | Percentage of Colonies Containing Indicated Myeloid Cell Lineages* . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MAC . | NEU . | MAST . | MAC NEU . | NEU MAST . | MAC MAST . | NEU MAC MAST . | NEU MAST MK . | NEU MAC MAST MK . | ||
| 1 | PU.1 null mice | 0 | 41 | 9 | 0 | 27 | 0 | 0 | 23 | 0 |
| Control mice | 4 | 0 | 29 | 8 | 8 | 8 | 13 | 0 | 30 | |
| 3 | PU.1 null mice | 0 | 42 | 0 | 0 | 29 | 0 | 0 | 29 | 0 |
| Control mice | 0 | 4 | 0 | 62 | 8 | 0 | 0 | 0 | 26 | |
Abbreviations: NEU, neutrophil; MAC, macrophage; MAST, mast cell; MK, megakaryocyte.
All cells from individual colonies were removed from representative clonogenic dishes (see Table 2), counted, and fixed to slides for staining and morphologic identification. Percentages of lineages calculated as number of colonies containing an observed myeloid cell type per total number of colonies.