Progenitor Cells Are Reduced in the Bone Marrow and Liver of PU.1 Null Neonates
| Experiment . | Cells From . | No. of Progenitors per Liver or Bone Marrow ×102 . | |
|---|---|---|---|
| CFC* . | HPP-CFC* . | ||
| 1 | PU.1 null bone marrow† | 0.26 ± 0.16 | 0.23 ± 0.13 |
| Control bone marrow | 22.48 ± 0.58 | 6.40 ± 0.25 | |
| PU.1 null liver | 4.51 ± 0.16 | 1.66 ± 0.03 | |
| Control liver | 40.22 ± 0.29 | 14.6 ± 0.80 | |
| 2 | PU.1 null bone marrow | 0.28‡ | 0.06‡ |
| Control bone marrow | 15.08 ± 0.33 | 5.56 ± 0.13 | |
| PU.1 null liver | 21.38 ± 0.95 | 11.87 ± 0.73 | |
| Control liver | 62.90 ± 19.14 | 39.24 ± 0.99 | |
| 3 | PU.1 null liver | 2.41 ± 0.35 | ND |
| Control liver | 52.00 ± 1.61 | ND | |
| Experiment . | Cells From . | No. of Progenitors per Liver or Bone Marrow ×102 . | |
|---|---|---|---|
| CFC* . | HPP-CFC* . | ||
| 1 | PU.1 null bone marrow† | 0.26 ± 0.16 | 0.23 ± 0.13 |
| Control bone marrow | 22.48 ± 0.58 | 6.40 ± 0.25 | |
| PU.1 null liver | 4.51 ± 0.16 | 1.66 ± 0.03 | |
| Control liver | 40.22 ± 0.29 | 14.6 ± 0.80 | |
| 2 | PU.1 null bone marrow | 0.28‡ | 0.06‡ |
| Control bone marrow | 15.08 ± 0.33 | 5.56 ± 0.13 | |
| PU.1 null liver | 21.38 ± 0.95 | 11.87 ± 0.73 | |
| Control liver | 62.90 ± 19.14 | 39.24 ± 0.99 | |
| 3 | PU.1 null liver | 2.41 ± 0.35 | ND |
| Control liver | 52.00 ± 1.61 | ND | |
*CFC includes all hematopoietic colonies of more than 50 cells, whereas HPP-CFC (colonies >0.5 mm) are as defined in text. Clonogenic assays were established in triplicate, except where noted. Values are reported as the mean ± SD. These experiments are representative of other evaluations of PU.1 null mice.
PU.1 null connotes mice with both alleles of the PU.1 gene disrupted, whereas control connotes mice with 1 or no alleles of PU.1 disrupted. Mice within an experiment were littermates. All groups, either control or PU.1 null, were composed of 2 to 5 mice.
Too few bone marrow cells were obtained from individual neonates; therefore, 3 bone marrow samples were pooled for analysis, yielding 1 clonogenic plate for progenitor analysis.