Hematopoietic Cells From PU.1 Null Mice Are Unable to Generate Colonies in Response to GM-CSF, G-CSF, or M-CSF
| Mouse . | No. of CFC (×101)/Input Hematopoietic Cells From Various Hematopoietic Compartments* . | |||
|---|---|---|---|---|
| Cytokine . | Phenotype . | Bone Marrow . | Spleen . | Liver . |
| IL-3 | Control-151 | ND | 22.3 ± 9.0 | 7.7 ± 4.5 |
| PU.1 null | ND | 19.7 ± 3.8 | 5.0 ± 3.6 | |
| GM-CSF | Control | 17.3 ± 7.5 | 20.7 ± 2.3 | 7.1 ± 1.1 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| G-CSF | Control | 4.0 ± 2.0 | 10.0 ± 2.6 | 1.3 ± 1.1 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| M-CSF | Control | 40.7 ± 4.9 | 21.7 ± 3.0 | 18.0 ± 5.6 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| Mouse . | No. of CFC (×101)/Input Hematopoietic Cells From Various Hematopoietic Compartments* . | |||
|---|---|---|---|---|
| Cytokine . | Phenotype . | Bone Marrow . | Spleen . | Liver . |
| IL-3 | Control-151 | ND | 22.3 ± 9.0 | 7.7 ± 4.5 |
| PU.1 null | ND | 19.7 ± 3.8 | 5.0 ± 3.6 | |
| GM-CSF | Control | 17.3 ± 7.5 | 20.7 ± 2.3 | 7.1 ± 1.1 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| G-CSF | Control | 4.0 ± 2.0 | 10.0 ± 2.6 | 1.3 ± 1.1 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| M-CSF | Control | 40.7 ± 4.9 | 21.7 ± 3.0 | 18.0 ± 5.6 |
| PU.1 null | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
*CFC includes all hematopoietic colonies of more than 50 cells; values are reported as the mean ± SD. Input cell numbers for clonogenic assay from neonates: bone marrow, 20,000 cells; spleen, 100,000 cells; liver, 50,000 cells. Two studies are shown; these experiments are representative of other evaluations of PU.1 null mice.
PU.1 null connotes mice with both alleles of the PU.1 gene disrupted, whereas control connotes mice with 1 or no alleles of PU.1 disrupted. Mice within an experiment were littermates. All groups, either control or PU.1 null, were composed of 1 to 3 mice.