Percentage of Cells in the Peritoneal Cavity and Bone Marrow of G-CSF−/− Mice and Wild-Type Mice Before and After Intraperitoneal Infection
| Infection . | Mice . | Peritoneal Cells . | Bone Marrow Cells . | ||
|---|---|---|---|---|---|
| Neutrophilic Granulocytes . | Macrophages/ Monocytes . | GR1+ . | F4/80+ . | ||
| None | GM-CSF−/− | 1 ± 1 | 88 ± 1 | 12 ± 3* | 11 ± 3 |
| Wild type | 2 ± 1 | 89 ± 1 | 48 ± 3 | 18 ± 7 | |
| 1 day | G-CSF−/− | 35 ± 8 | 57 ± 10 | 16 ± 4* | 8 ± 4 |
| Wild type | 27 ± 7 | 70 ± 4 | 46 ± 8 | 23 ± 17 | |
| 3 days | G-CSF−/− | 15 ± 10 | 70 ± 5 | 30 ± 7* | 11 ± 3 |
| Wild type | 10 ± 4 | 76 ± 8 | 54 ± 4 | 19 ± 9 | |
| Infection . | Mice . | Peritoneal Cells . | Bone Marrow Cells . | ||
|---|---|---|---|---|---|
| Neutrophilic Granulocytes . | Macrophages/ Monocytes . | GR1+ . | F4/80+ . | ||
| None | GM-CSF−/− | 1 ± 1 | 88 ± 1 | 12 ± 3* | 11 ± 3 |
| Wild type | 2 ± 1 | 89 ± 1 | 48 ± 3 | 18 ± 7 | |
| 1 day | G-CSF−/− | 35 ± 8 | 57 ± 10 | 16 ± 4* | 8 ± 4 |
| Wild type | 27 ± 7 | 70 ± 4 | 46 ± 8 | 23 ± 17 | |
| 3 days | G-CSF−/− | 15 ± 10 | 70 ± 5 | 30 ± 7* | 11 ± 3 |
| Wild type | 10 ± 4 | 76 ± 8 | 54 ± 4 | 19 ± 9 | |
Mice were infected with 2 × 104Listeriaintraperitoneally and groups of five mice were killed at day 1 or 3 postinfection, or without infection. Peritoneal cells and bone marrow cells were prepared from individual animals. Peritoneal cells were assessed microscopically, while bone marrow cells were analyzed by FACS.
P < .001 comparing G-CSF−/− and wild-type mice. Other differences between G-CSF−/− and wild-type mice were not significant. The results are typical of four separate experiments.