Percentage of Cells in the Peritoneal Cavity and Bone Marrow of GM-CSF−/− Mice and Wild-Type Mice Before and After Intraperitoneal Infection
| Infection . | Mice . | Peritoneal Cells . | Bone Marrow Cells . | ||
|---|---|---|---|---|---|
| Neutrophilic Granulocytes . | Macrophages/ Monocytes . | GR1+ . | F4/80+ . | ||
| None | GM-CSF−/− | 1 ± 1 | 81 ± 2 | 58 ± 3 | 12 ± 2 |
| Wild type | 1 ± 1 | 81 ± 3 | 52 ± 5 | 8 ± 1 | |
| 1 day | GM-CSF−/− | 57 ± 11 | 41 ± 13 | 60 ± 7-150 | 7 ± 2 |
| Wild type | 56 ± 8 | 38 ± 10 | 48 ± 6 | 8 ± 2 | |
| 3 days | GM-CSF−/− | 34 ± 6 | 56 ± 6-151 | 62 ± 10 | 10 ± 2 |
| Wild type | 22 ± 8 | 73 ± 9 | 68 ± 2 | 9 ± 2 | |
| Infection . | Mice . | Peritoneal Cells . | Bone Marrow Cells . | ||
|---|---|---|---|---|---|
| Neutrophilic Granulocytes . | Macrophages/ Monocytes . | GR1+ . | F4/80+ . | ||
| None | GM-CSF−/− | 1 ± 1 | 81 ± 2 | 58 ± 3 | 12 ± 2 |
| Wild type | 1 ± 1 | 81 ± 3 | 52 ± 5 | 8 ± 1 | |
| 1 day | GM-CSF−/− | 57 ± 11 | 41 ± 13 | 60 ± 7-150 | 7 ± 2 |
| Wild type | 56 ± 8 | 38 ± 10 | 48 ± 6 | 8 ± 2 | |
| 3 days | GM-CSF−/− | 34 ± 6 | 56 ± 6-151 | 62 ± 10 | 10 ± 2 |
| Wild type | 22 ± 8 | 73 ± 9 | 68 ± 2 | 9 ± 2 | |
Mice were infected with 2 × 105Listeriaintraperitoneally and groups of five mice were killed at day 1 or 3 postinfection, or without infection. Peritoneal cells and bone marrow cells were prepared from individual animals. Peritoneal cells were assessed microscopically, while bone marrow cells were analyzed by FACS. The results are typical of four separate experiments.
P < .05 by Student's t-test.
P < .01 by Student's t-test.