Table 1. Stimulation of PKC... | American Society of Hematology

Table 1.

Stimulation of PKC Translocation by TPO in c-Mpl–Expressing Cells

Cell Line c-Mpl Expression^-150	UT-7 None		UT-7/mpl 5.3 Low		UT-7/mpl 5.1 Intermediate		UT-7/mpl 1101C High		CMK ND
Cell Line c-Mpl Expression^-150	Cyto	Mem	Cyto	Mem	Cyto	Mem	Cyto	Mem	Cyto	Mem
Control^-151	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
7 ng/mL TPO	1.00^-152	1.03^-152	0.90^-153	1.31^-153	0.87^-153	1.57^-153	0.80^-153	2.54^-153	0.80^-153	2.27^-153
40 ng/mL TPO	0.96^-152	1.02^-152	0.86^-153	1.43^-153	0.80^-153	2.33^-155	0.78^-153	2.91^-155	0.73^-153	2.40^-155

Cell Line c-Mpl Expression^-150	UT-7 None		UT-7/mpl 5.3 Low		UT-7/mpl 5.1 Intermediate		UT-7/mpl 1101C High		CMK ND
Cell Line c-Mpl Expression^-150	Cyto	Mem	Cyto	Mem	Cyto	Mem	Cyto	Mem	Cyto	Mem
Control^-151	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
7 ng/mL TPO	1.00^-152	1.03^-152	0.90^-153	1.31^-153	0.87^-153	1.57^-153	0.80^-153	2.54^-153	0.80^-153	2.27^-153
40 ng/mL TPO	0.96^-152	1.02^-152	0.86^-153	1.43^-153	0.80^-153	2.33^-155	0.78^-153	2.91^-155	0.73^-153	2.40^-155

Cells were treated with the indicated concentrations of TPO for 15 minutes and then PKC activity was measured in the cytosolic and membrane fractions as described in Materials and Methods. PKC activity is expressed relative to the level present in unstimulated cells (control). The data represent the mean of two determinations from one representative experiment.

Abbreviations: ND, not determined; Cyto, cytosolic; Mem, membrane.

F0-150

Relative levels of c-Mpl expression were determined by flow cytometry as described in Materials and Methods.

F0-151

For unstimulated cells, the levels of PKC in the membrane and cytosolic fractions of the different UT-7 cell clones ranged from 1.9 to 2.5 U/10⁵ cells and from 14.5 to 22.7 U/10⁵cells, respectively. In CMK cells these levels were 1.7 U/10⁵ cells and 10.1 U/10⁵ cells, respectively.

P > .05.

P < .05.

P < .01.

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