IL-3 Expression During Delayed-Type Hypersensitivity Responses
| Antigen . | Time After Priming (h) . | |||||
|---|---|---|---|---|---|---|
| 0 . | 1 . | 12 . | 24 . | 48 . | 96 . | |
| None | 0-150/8-151 | — | — | 0/8-152 | — | — |
| Vehicle only-153 | — | — | 0/5 | 0/5 | — | — |
| Oxazolone | — | 4/4 | 4/4 | 8/10 | 4/10 | 3/8 |
| Tumor cells-155 | — | — | — | 0/4 | 0/4 | — |
| Antigen . | Time After Priming (h) . | |||||
|---|---|---|---|---|---|---|
| 0 . | 1 . | 12 . | 24 . | 48 . | 96 . | |
| None | 0-150/8-151 | — | — | 0/8-152 | — | — |
| Vehicle only-153 | — | — | 0/5 | 0/5 | — | — |
| Oxazolone | — | 4/4 | 4/4 | 8/10 | 4/10 | 3/8 |
| Tumor cells-155 | — | — | — | 0/4 | 0/4 | — |
Wild-type mice (BALB/c) were sensitized with the indicated antigen, and the presence of IL-3 transcripts at varying times in the skin at the priming site was evaluated by RT-PCR. No message was detectable in IL-3–deficient mice.
Number of mice positive for IL-3 transcripts.
Number of mice tested.
Shaved only.
Acetone/olive oil.
Irradiated RENCA carcinoma cells (3,300 rads).