Table 3.

SCF Induces c-kit Receptor Dimerization in a Dose-Dependent Manner

Concentration of SCF (ng/mL) FITC Mean Fluorescence Intensity Cy3 Mean Fluorescence IntensityAcceptor/ Donor Ratio
Experiment 1  0  89.7 99.6  1.11  
 1  88.2  100.1  1.13  
 3 87.6  101.0  1.15  
 10  85.6  109.3  1.28 
 30  88.9  131.2  1.48  
 100  77.4  132.6 1.71  
 300  80.7  137.2  1.70  
 1000  76.4 127.1  1.66  
Experiment 2  0  219  320  1.46 
 2  227  341  1.50  
 10  210  346  1.65 
 50  179  398  2.22  
 250  170  403 2.37 
Concentration of SCF (ng/mL) FITC Mean Fluorescence Intensity Cy3 Mean Fluorescence IntensityAcceptor/ Donor Ratio
Experiment 1  0  89.7 99.6  1.11  
 1  88.2  100.1  1.13  
 3 87.6  101.0  1.15  
 10  85.6  109.3  1.28 
 30  88.9  131.2  1.48  
 100  77.4  132.6 1.71  
 300  80.7  137.2  1.70  
 1000  76.4 127.1  1.66  
Experiment 2  0  219  320  1.46 
 2  227  341  1.50  
 10  210  346  1.65 
 50  179  398  2.22  
 250  170  403 2.37 

MO7e cells were labeled with 104D2-FITC plus 104D2-Cy3 as described in Table 2, then incubated with a range of concentrations of SCF for 20 minutes at 37°C, fixed, and analyzed by flow cytometry.

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