SCF Induces c-kit Receptor Dimerization in a Dose-Dependent Manner
| . | Concentration of SCF (ng/mL) . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| Experiment 1 | 0 | 89.7 | 99.6 | 1.11 |
| 1 | 88.2 | 100.1 | 1.13 | |
| 3 | 87.6 | 101.0 | 1.15 | |
| 10 | 85.6 | 109.3 | 1.28 | |
| 30 | 88.9 | 131.2 | 1.48 | |
| 100 | 77.4 | 132.6 | 1.71 | |
| 300 | 80.7 | 137.2 | 1.70 | |
| 1000 | 76.4 | 127.1 | 1.66 | |
| Experiment 2 | 0 | 219 | 320 | 1.46 |
| 2 | 227 | 341 | 1.50 | |
| 10 | 210 | 346 | 1.65 | |
| 50 | 179 | 398 | 2.22 | |
| 250 | 170 | 403 | 2.37 |
| . | Concentration of SCF (ng/mL) . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| Experiment 1 | 0 | 89.7 | 99.6 | 1.11 |
| 1 | 88.2 | 100.1 | 1.13 | |
| 3 | 87.6 | 101.0 | 1.15 | |
| 10 | 85.6 | 109.3 | 1.28 | |
| 30 | 88.9 | 131.2 | 1.48 | |
| 100 | 77.4 | 132.6 | 1.71 | |
| 300 | 80.7 | 137.2 | 1.70 | |
| 1000 | 76.4 | 127.1 | 1.66 | |
| Experiment 2 | 0 | 219 | 320 | 1.46 |
| 2 | 227 | 341 | 1.50 | |
| 10 | 210 | 346 | 1.65 | |
| 50 | 179 | 398 | 2.22 | |
| 250 | 170 | 403 | 2.37 |
MO7e cells were labeled with 104D2-FITC plus 104D2-Cy3 as described in Table 2, then incubated with a range of concentrations of SCF for 20 minutes at 37°C, fixed, and analyzed by flow cytometry.