SCF-Induced Dimerization of the c-kit Receptor Is Detectable by FRET
| Temperature . | Growth Factor . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| 37°C | 0 | 180 | 247 | 1.37 |
| SCF | 85 | 314 | 3.69 | |
| GM-CSF | 174 | 251 | 1.44 | |
| IL-3 | 185 | 244 | 1.32 | |
| Epo | 172 | 236 | 1.37 | |
| 4°C | 0 | 203 | 193 | 0.95 |
| SCF | 196 | 213 | 1.10 |
| Temperature . | Growth Factor . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| 37°C | 0 | 180 | 247 | 1.37 |
| SCF | 85 | 314 | 3.69 | |
| GM-CSF | 174 | 251 | 1.44 | |
| IL-3 | 185 | 244 | 1.32 | |
| Epo | 172 | 236 | 1.37 | |
| 4°C | 0 | 203 | 193 | 0.95 |
| SCF | 196 | 213 | 1.10 |
MO7e cells were labeled with a mixture of 104D2-FITC plus 104D2-Cy3 for 1 hour at 4°C, then incubated with no added growth factor, SCF (100 ng/mL), GM-CSF (2.5 ng/mL), IL-3 (2.5 ng/mL), or Epo (4 U/mL) for 1 hour at 37°C, or with no added growth factor or SCF (100 ng/mL) for 1 hour at 4°C. The cells were fixed in 1% paraformaldehyde, and analyzed in a Coulter Epics Elite Flow Cytometer to detect FITC fluorescence at 505 to 545 nm, and Cy3 fluorescence above 590 nm.