Expression Levels of Normal and Mutated Protein C From Stably Transfected CHO Cells
| Proteins . | Intracellular Protein C Ag (ng/1 × 106 cells/24 h) n = 2 . | Secreted Protein C Ag (ng/1 × 106 cells/24 h) n = 3 . | Relative Activity of Secreted Protein C (%) n = 3 . |
|---|---|---|---|
| PCN | 85.2 ± 5.5 | ND | NE |
| PCN444 | 9.8 ± 0.2 | ND | NE |
| PCN425 | 9.3 ± 2.8 | ND | NE |
| PCN388 | 7.4 ± 0.7 | ND | NE |
| PCN380 | 22.9 ± 0.3 | ND | NE |
| wtPC | 151.7 ± 0.4 | 707.2 ± 21.3 | 94.3 ± 0.8 |
| PC416 | 71.3 ± 9.2 | 557.5 ± 73.4* | 88.4 ± 5.1 |
| PC413 | 62.2 ± 3.7 | 528.9 ± 97.8* | 96.5 ± 10.0 |
| PC410 | 58.5 ± 0.1 | 454.7 ± 82.6* | 92.5 ± 1.0 |
| PC409 | 47.6 ± 2.1 | 435.0 ± 72.2* | 93.2 ± 5.8 |
| PC408 | 21.2 ± 0.5 | ND | NE |
| PC407 | 7.3 ± 0.5 | ND | NE |
| PC406 | 16.5 ± 0.5 | ND | NE |
| PC403 | 7.8 ± 0.5 | ND | NE |
| PC401 | 12.7 ± 0.7 | ND | NE |
| Proteins . | Intracellular Protein C Ag (ng/1 × 106 cells/24 h) n = 2 . | Secreted Protein C Ag (ng/1 × 106 cells/24 h) n = 3 . | Relative Activity of Secreted Protein C (%) n = 3 . |
|---|---|---|---|
| PCN | 85.2 ± 5.5 | ND | NE |
| PCN444 | 9.8 ± 0.2 | ND | NE |
| PCN425 | 9.3 ± 2.8 | ND | NE |
| PCN388 | 7.4 ± 0.7 | ND | NE |
| PCN380 | 22.9 ± 0.3 | ND | NE |
| wtPC | 151.7 ± 0.4 | 707.2 ± 21.3 | 94.3 ± 0.8 |
| PC416 | 71.3 ± 9.2 | 557.5 ± 73.4* | 88.4 ± 5.1 |
| PC413 | 62.2 ± 3.7 | 528.9 ± 97.8* | 96.5 ± 10.0 |
| PC410 | 58.5 ± 0.1 | 454.7 ± 82.6* | 92.5 ± 1.0 |
| PC409 | 47.6 ± 2.1 | 435.0 ± 72.2* | 93.2 ± 5.8 |
| PC408 | 21.2 ± 0.5 | ND | NE |
| PC407 | 7.3 ± 0.5 | ND | NE |
| PC406 | 16.5 ± 0.5 | ND | NE |
| PC403 | 7.8 ± 0.5 | ND | NE |
| PC401 | 12.7 ± 0.7 | ND | NE |
The antigen and relative activity of protein C were measured as described previously.5 Statistical analysis of secreted protein C antigen and activity levels were performed between wtPC and other secreted proteins by Mann-Whitney test.
Denotes a P value of <.05.
Abbreviations: ND, not detected; NE, not examined.