The Oligonucleotides Used to Construct the Deletion Mutants of PCN and wtPC
| a. PCN Oligonucleotides . | Proteins . |
|---|---|
| Sense: | |
| C1 (5′-GTGTCTGAGAACATGCTGTGT-3′, nucleotides 1238-1258 in pBPCN) | |
| Antisense: | |
| N380 (5′-AAGGAGCCCACAGCCCTCta-3′, nucleotides 1383-1364 in pBPCN) | PCN380 |
| N388 (5′-CCGTttaTGTGAAGGAGCCCACA-3′, nucleotides 1394-1372 in pBPCN) | PCN388 |
| N425 (5′-ttaCCTGCAGGGAGGGTCGC-3′, nucleotides 1501-1482 in pBPCN) | PCN425 |
| N444 (5′-AGCAGtCaGGTGTGCTTGTT-3′, nucleotides 1563-1544 in pBPCN) | PCN444 |
| b. wtPC Oligonucleotides | Proteins |
| Sense: | |
| C1 (5′-GTGTCTGAGAACATGCTGTGT-3′, nucleotides 1238-1258 in pBPC) | |
| Antisense: | |
| C401 (5′-CCCATGGATtCAGTCGAGGTA-3′, nucleotides 1438-1418 in pBPC) | PC401 |
| C403 (5′-CTGATGTGCCCTtaGATCCAGT-3′, nucleotides 1446-1425 in pBPC) | PC403 |
| C406 (5′-CTTGTCTCTctaGTGCCCATGG-3′, nucleotides 1453-1432 in pBPC) | PC406 |
| C407 (5′-TCTCaGATGTGCCCATGGATCC-3′, nucleotides 1449-1428 in pBPC) | PC407 |
| C408 (5′-GGCTTCCTTcTaTCTGATGTGC-3′, nucleotides 1459-1438 in pBPC) | PC408 |
| C409 (5′-GGCTTCcTaGTCTCTGATGTG-3′, nucleotides 1459-1439 in pBPC) | PC409 |
| C410 (5′-GGGCTTaCTTGTCTCTGATGT-3′, nucleotides 1460-1440 in pBPC) | PC410 |
| C413 (5′-AGCTCTTTCTaGGGGGCTTCC-3′, nucleotides 1472-1453 in pBPC) | PC413 |
| C416 (5′-GCTAAGGTGCtCAGCTCTTCT-3′, nucleotides 1484-1464 in pBPC) | PC416 |
| a. PCN Oligonucleotides . | Proteins . |
|---|---|
| Sense: | |
| C1 (5′-GTGTCTGAGAACATGCTGTGT-3′, nucleotides 1238-1258 in pBPCN) | |
| Antisense: | |
| N380 (5′-AAGGAGCCCACAGCCCTCta-3′, nucleotides 1383-1364 in pBPCN) | PCN380 |
| N388 (5′-CCGTttaTGTGAAGGAGCCCACA-3′, nucleotides 1394-1372 in pBPCN) | PCN388 |
| N425 (5′-ttaCCTGCAGGGAGGGTCGC-3′, nucleotides 1501-1482 in pBPCN) | PCN425 |
| N444 (5′-AGCAGtCaGGTGTGCTTGTT-3′, nucleotides 1563-1544 in pBPCN) | PCN444 |
| b. wtPC Oligonucleotides | Proteins |
| Sense: | |
| C1 (5′-GTGTCTGAGAACATGCTGTGT-3′, nucleotides 1238-1258 in pBPC) | |
| Antisense: | |
| C401 (5′-CCCATGGATtCAGTCGAGGTA-3′, nucleotides 1438-1418 in pBPC) | PC401 |
| C403 (5′-CTGATGTGCCCTtaGATCCAGT-3′, nucleotides 1446-1425 in pBPC) | PC403 |
| C406 (5′-CTTGTCTCTctaGTGCCCATGG-3′, nucleotides 1453-1432 in pBPC) | PC406 |
| C407 (5′-TCTCaGATGTGCCCATGGATCC-3′, nucleotides 1449-1428 in pBPC) | PC407 |
| C408 (5′-GGCTTCCTTcTaTCTGATGTGC-3′, nucleotides 1459-1438 in pBPC) | PC408 |
| C409 (5′-GGCTTCcTaGTCTCTGATGTG-3′, nucleotides 1459-1439 in pBPC) | PC409 |
| C410 (5′-GGGCTTaCTTGTCTCTGATGT-3′, nucleotides 1460-1440 in pBPC) | PC410 |
| C413 (5′-AGCTCTTTCTaGGGGGCTTCC-3′, nucleotides 1472-1453 in pBPC) | PC413 |
| C416 (5′-GCTAAGGTGCtCAGCTCTTCT-3′, nucleotides 1484-1464 in pBPC) | PC416 |
The oligonucleotides used to construct the deletion mutants of PCN and wtPC are shown. The mutant bases are indicated by lower case letters.