Table 2. FcRIα Upregulation in Cultured... | American Society of Hematology

Table 2.

FcRIα Upregulation in Cultured Basophils

Effects of IgG on FcεRIα expression in cultured basophils
Experiment No.	Control	IgG^*	IgG^†	IgE^‡	IgE + IgG
1	30¶	32		67
2	24	25		96

Average of
1 and 2	27 ± 4	29 ± 5		82 ± 21

3	22		26	65	63
4	40		40	88	79

Average of
3 and 4	31 ± 13		33 ± 10	77 ± 16	71 ± 11

Effects of IgG on FcεRIα expression in cultured basophils
Experiment No.	Control	IgG^*	IgG^†	IgE^‡	IgE + IgG
1	30¶	32		67
2	24	25		96

Average of
1 and 2	27 ± 4	29 ± 5		82 ± 21

3	22		26	65	63
4	40		40	88	79

Average of
3 and 4	31 ± 13		33 ± 10	77 ± 16	71 ± 11

Effects of dimeric IgE on FcεRIα expression in cultured basophils
Experiment No.	Control	IgE^‡	Dimer^1-153	IgE^‡ + D@50	IgE^‡ + D@500
1	53	83	48
2	40	88	41
3	42	107	48	90	82

Average of
1 to 3	45 ± 7	93 ± 13	46 ± 4

Effects of dimeric IgE on FcεRIα expression in cultured basophils
Experiment No.	Control	IgE^‡	Dimer^1-153	IgE^‡ + D@50	IgE^‡ + D@500
1	53	83	48
2	40	88	41
3	42	107	48	90	82

Average of
1 to 3	45 ± 7	93 ± 13	46 ± 4

Upregulation of FcεRIα in high-purity basophils cultured with IgE
Experiment No.	Purity	Control	IgE^‡	Fold Upregulation	Expected^1-155
1	97	35	88	2.7	2.4
2	90	12	57	4.8	4.5

Upregulation of FcεRIα in high-purity basophils cultured with IgE
Experiment No.	Purity	Control	IgE^‡	Fold Upregulation	Expected^1-155
1	97	35	88	2.7	2.4
2	90	12	57	4.8	4.5

Basophils were cultured with the various listed agents for 7 days and expression of FcεRIα was determined by 22E7 binding using flow cytometry. Data for individual experiments are shown; average values were not calculated for the experiments in the third section.

IgG concentration = 1 μg/mL.

†

IgG concentration = 1 mg/mL.

‡

IgE = PS myeloma at 500 ng/mL.

F1-153

Dimer = covalently linked dimeric IgE at 500 ng/mL, D@50 = dimeric IgE at 50 ng/mL, D@500 = dimeric IgE at 500 ng/mL.

¶Listed values are net fluorescence units for monoclonal antibody 22E7 binding as determined by flow cytometry.

F1-155

Expected values are calculated from the starting FcεRIα flow-cytometric density using the relationship shown in Fig5.

View Large

This Feature Is Available To Subscribers Only