IRP-1 and IRP-2 Activity and Ferritin Content in Cytokine-Treated J774 Cells
| . | IRP-1 (%) . | IRP-2 (%) . | FT-H (ng/mg prot) . | FT-L (ng/mg prot) . |
|---|---|---|---|---|
| Control | 100 | 100 | 218 ± 66 | 80 ± 21 |
| IFN-γ/LPS 4 h | 132 ± 14-150 | 37 ± 12-150 | — | — |
| IFN-γ/LPS 24 h | 141 ± 9-150 | 27 ± 6-150 | 489 ± 93-150 | 102 ± 47 |
| SNAP 24 h | 136 ± 11-150 | 29 ± 5-150 | 467 ± 88-150 | 100 ± 83 |
| . | IRP-1 (%) . | IRP-2 (%) . | FT-H (ng/mg prot) . | FT-L (ng/mg prot) . |
|---|---|---|---|---|
| Control | 100 | 100 | 218 ± 66 | 80 ± 21 |
| IFN-γ/LPS 4 h | 132 ± 14-150 | 37 ± 12-150 | — | — |
| IFN-γ/LPS 24 h | 141 ± 9-150 | 27 ± 6-150 | 489 ± 93-150 | 102 ± 47 |
| SNAP 24 h | 136 ± 11-150 | 29 ± 5-150 | 467 ± 88-150 | 100 ± 83 |
J774 cells were left untreated (control) or treated for 4 or 24 hours with cytokines (100 U/mL IFN-γ plus 1 μg/mL LPS) or treated with 0.5 mmol/L SNAP for 24 hours. IRP activity and ferritin content was determined as described in Materials and Methods. Values represent mean ± SD of 10 (IFN-γ/LPS) and 7 (SNAP) experiments. Values of IRP activity are given as a percentage of control.
P < .001 v controls.