Table 1.

Supplemental Cytokines in the Presence SCM and FL Stimulates the Expression of CD3γ, CD3δ, CD3ζ, and CD3 mRNA in Purified Human CD34+Lin−DR− Cells

Conditions-151DaymRNA Positive/Total Samples Tested-150
CD3γCD3δCD3ζCD3εβ-actin
CD34+Lin−DR−(fresh)  0  0/16  0/16  0/16  14/16  16/16 
IL-2/PHA  14  0/4  0/4  0/4  1/4  4/4 
Cytokines + SCM  7  1/20  4/20  0/20  20/20 20/20  
Cytokines  14  0/20  3/20  17/20  20/20 20/20  
Cytokines + SCM  14  11/20  11/20  16/20 20/20  20/20  
Cytokines + SCM + FL  14  5/5  5/5 5/5  5/5  5/5 
Conditions-151DaymRNA Positive/Total Samples Tested-150
CD3γCD3δCD3ζCD3εβ-actin
CD34+Lin−DR−(fresh)  0  0/16  0/16  0/16  14/16  16/16 
IL-2/PHA  14  0/4  0/4  0/4  1/4  4/4 
Cytokines + SCM  7  1/20  4/20  0/20  20/20 20/20  
Cytokines  14  0/20  3/20  17/20  20/20 20/20  
Cytokines + SCM  14  11/20  11/20  16/20 20/20  20/20  
Cytokines + SCM + FL  14  5/5  5/5 5/5  5/5  5/5 

Abbreviations: SCM, stroma-conditioned media; FL, FLT-3 ligand.

F0-150

Data represent the number of transcript-positive samples detected by Southern blotting over total number of replicate reactions.

F0-151

Cytokines: IL-2, IL-3, IL-7, and SCF.

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