HLA Restriction of BCR/ABLb3a2 Peptide P2-Specific T-Cell Clone (P218) by Using Monocytes Pulsed With P2
| Targets . | HLA-A . | HLA-B . | HLA-C . | % Specific Lysis αA2/A24MoAbs . | |
|---|---|---|---|---|---|
| (−) . | (+) . | ||||
| HLA-A24 positive monocytes | |||||
| NG (Auto) | 24 | 52/59 | 1 | 23 ± 3-150 | NT |
| JN (Allo) | 24 | 51/61 | 10 | 25 ± 2-150 | NT |
| NY (Allo) | 24 | 52/61 | 10 | 20 ± 3-150 | 0 |
| HLA-A24 negative monocytes | |||||
| OY (Allo) | 2/11 | 13/51 | 10 | 3 ± 2 | 1 ± 1 |
| KD (Allo) | 2/26 | 7/46 | 1/7 | 1 ± 1 | 0 ± 1 |
| Targets . | HLA-A . | HLA-B . | HLA-C . | % Specific Lysis αA2/A24MoAbs . | |
|---|---|---|---|---|---|
| (−) . | (+) . | ||||
| HLA-A24 positive monocytes | |||||
| NG (Auto) | 24 | 52/59 | 1 | 23 ± 3-150 | NT |
| JN (Allo) | 24 | 51/61 | 10 | 25 ± 2-150 | NT |
| NY (Allo) | 24 | 52/61 | 10 | 20 ± 3-150 | 0 |
| HLA-A24 negative monocytes | |||||
| OY (Allo) | 2/11 | 13/51 | 10 | 3 ± 2 | 1 ± 1 |
| KD (Allo) | 2/26 | 7/46 | 1/7 | 1 ± 1 | 0 ± 1 |
Results are expressed as % specific lysis at an E:T ratio of 10:1.
Abbreviation: NT, not tested.
Significant lysis P < .0002.