Table 4. Engraftment of Human Cells in... | American Society of Hematology

Table 4.

Engraftment of Human Cells in Secondary Recipient Fetuses

Experiment No.	Animal No.	No. of Cells Injected (×10⁶)	% CD45⁺ Cells in BM	Human/Total Colonies (%)		Chimeric/ Total Animals
Experiment No.	Animal No.	No. of Cells Injected (×10⁶)	% CD45⁺ Cells in BM	Mix	GM	Chimeric/ Total Animals
[Fresh Cells]
1	606	3.0	1.3	4.8	9.7	3/4
	607	3.0	3.1	0	2.8
	612	0.4	<0.1^3-150	ND	ND
	613	0.4	4.8	3.2	4.3
2	40	0.6	7.2	4.4	6.8	2/3
	41	0.6	4.3	3.2	5.3
	42	0.6	<0.1^3-150	ND	ND
3	20	1.2	<0.1^3-150	ND	ND	5/6
	21	1.2	1.4	0	5.6
	22	1.2	2.1	0	4.2
	23	1.2	1.1	3.6	5.6
	24	1.2	1.3	2.9	8.1
	25	1.2	1.3	1.6	5.0
[Cultured Cells]
1	801-807	2.1	<0.1^3-150	ND	ND	0/12
	809-813	2.2	<0.1^3-150	ND	ND
2	61, 62, 64, 66, 67	2.3	<0.1^3-150	ND	ND	2/7
	63	2.3	1.3	0	2.5
	65	2.3	0.9	1.8	6.3
3	84-87	1.5	<0.1^3-150	ND	ND	1/8
	88, 89, 91	3.4	<0.1^3-150	ND	ND
	90	3.4	0.3	2.3	3.8

Experiment No.	Animal No.	No. of Cells Injected (×10⁶)	% CD45⁺ Cells in BM	Human/Total Colonies (%)		Chimeric/ Total Animals
Experiment No.	Animal No.	No. of Cells Injected (×10⁶)	% CD45⁺ Cells in BM	Mix	GM	Chimeric/ Total Animals
[Fresh Cells]
1	606	3.0	1.3	4.8	9.7	3/4
	607	3.0	3.1	0	2.8
	612	0.4	<0.1^3-150	ND	ND
	613	0.4	4.8	3.2	4.3
2	40	0.6	7.2	4.4	6.8	2/3
	41	0.6	4.3	3.2	5.3
	42	0.6	<0.1^3-150	ND	ND
3	20	1.2	<0.1^3-150	ND	ND	5/6
	21	1.2	1.4	0	5.6
	22	1.2	2.1	0	4.2
	23	1.2	1.1	3.6	5.6
	24	1.2	1.3	2.9	8.1
	25	1.2	1.3	1.6	5.0
[Cultured Cells]
1	801-807	2.1	<0.1^3-150	ND	ND	0/12
	809-813	2.2	<0.1^3-150	ND	ND
2	61, 62, 64, 66, 67	2.3	<0.1^3-150	ND	ND	2/7
	63	2.3	1.3	0	2.5
	65	2.3	0.9	1.8	6.3
3	84-87	1.5	<0.1^3-150	ND	ND	1/8
	88, 89, 91	3.4	<0.1^3-150	ND	ND
	90	3.4	0.3	2.3	3.8

Abbreviation: ND, not done.

CD45⁺ cells that were less than 0.1% were considered negative.