Effect of Various Concentration Gradients of SN on Eosinophil Migration
| . | . | SN [mol/L], Upper Chamber . | |||
|---|---|---|---|---|---|
| Medium . | 10−11 . | 10−9 . | 10−7 . | ||
| Medium | 1.000 ± 0.000 | 1.059 ± 0.030 | 1.108 ± 0.052 | 1.149 ± 0.072* | |
| SN [mol/L], lower chamber | 10−11 | 1.178 ± 0.076* | 0.992 ± 0.049 | 1.098 ± 0.021 | 1.116 ± 0.056 |
| 10−9 | 1.332 ± 0.034† | 1.187 ± 0.047* | 1.099 ± 0.037 | 1.119 ± 0.060 | |
| 10−7 | 1.535 ± 0.018‡ | 1.383 ± 0.079† | 1.030 ± 0.059 | 1.110 ± 0.047 | |
| . | . | SN [mol/L], Upper Chamber . | |||
|---|---|---|---|---|---|
| Medium . | 10−11 . | 10−9 . | 10−7 . | ||
| Medium | 1.000 ± 0.000 | 1.059 ± 0.030 | 1.108 ± 0.052 | 1.149 ± 0.072* | |
| SN [mol/L], lower chamber | 10−11 | 1.178 ± 0.076* | 0.992 ± 0.049 | 1.098 ± 0.021 | 1.116 ± 0.056 |
| 10−9 | 1.332 ± 0.034† | 1.187 ± 0.047* | 1.099 ± 0.037 | 1.119 ± 0.060 | |
| 10−7 | 1.535 ± 0.018‡ | 1.383 ± 0.079† | 1.030 ± 0.059 | 1.110 ± 0.047 | |
Checkerboard analysis of eosinophil migration. Different concentrations of SN were added to the upper and/or lower compartment of chemotaxis chambers. RPMI 1640/0.5% BSA served as control medium. After a migration period of 60 minutes at 37°C (humidified atmosphere 5% CO2) the chemotaxis index of migration into the nitrocellulose filter was measured microscopically. “Chemotaxis Index” represents the ratio between the distance cells migrate toward test substances and that toward control medium (mean ± SEM, three independent experiments each performed in replicates of three). Statistical analysis: Mann-Whitney U-test: *P < .05; †P < .01 v medium in upper and lower chamber, ‡P < .01 v 0.1 μmol/L SN in upper and medium in lower chamber.