Table 2. Engraftment of... | American Society of Hematology

Table 2.

Engraftment of Separated/Selected Cells Into Minimally Irradiated Hosts: Lineage-Depleted Male Marrow Cells Into Female Hosts

	LineageDepleted	Hoechst^Low Rhodamine^low
No. of cells infused	0.979 × 10⁶	3,588
Engraftment (%)
0 cGy	4.23 ± 0.46	2.57 ± 0.59
20 cGy	5.90 ± 1.47
50 cGy	13.57 ± 2.67
100 cGy	23.36 ± 2.67	27.4 ± 7.55

	LineageDepleted	Hoechst^Low Rhodamine^low
No. of cells infused	0.979 × 10⁶	3,588
Engraftment (%)
0 cGy	4.23 ± 0.46	2.57 ± 0.59
20 cGy	5.90 ± 1.47
50 cGy	13.57 ± 2.67
100 cGy	23.36 ± 2.67	27.4 ± 7.55

A total of .979 × 10⁶ cells derived from 59.6 × 10⁶ starting low-density marrow cells were infused into female hosts. At 50 cGy, the percentage of male engraftment was increased compared with 0 cGy in marrow, spleen, and thymus (only marrow shown); 5 mice were included in each group (P < .01). This effect was shown also in animals treated with 100 cGy compared with 0 cGy (P < .01). For Hoechst^low Rhodamine^low, a total of 3,588 separated cells derived from 993 × 10⁶ starting unseparated male BALB/c cells were infused into female hosts. At 100 cGy, male marrow engraftment was increased compared with 0 cGy in marrow, spleen, and thymus (only marrow shown).

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