Induction of Spontaneous Monocytic Differentiation in M1 Cells Transfected With WT1 +KTS Isoforms
| Cell Type . | M1 (%) . | M1.Neo (%) . | M1.WT1.1 (+/+) (%) . | M1.WT1.2 (−/+) (%) . | M1.WT1.3 (+/−) (%) . | M1.WT1.4 (−/−) (%) . |
|---|---|---|---|---|---|---|
| Monoblast | 100 | 98 | 42 | 41 | 92 | 83 |
| Promonocyte | 0 | 2 | 26 | 33 | 6 | 15 |
| Monocyte | 0 | 0 | 30 | 18 | 2 | 2 |
| Macrophage | 0 | 0 | 2 | 8 | 0 | 0 |
| Cell Type . | M1 (%) . | M1.Neo (%) . | M1.WT1.1 (+/+) (%) . | M1.WT1.2 (−/+) (%) . | M1.WT1.3 (+/−) (%) . | M1.WT1.4 (−/−) (%) . |
|---|---|---|---|---|---|---|
| Monoblast | 100 | 98 | 42 | 41 | 92 | 83 |
| Promonocyte | 0 | 2 | 26 | 33 | 6 | 15 |
| Monocyte | 0 | 0 | 30 | 18 | 2 | 2 |
| Macrophage | 0 | 0 | 2 | 8 | 0 | 0 |
M1 cells stably transfected with retroviral vectors containing cDNAs encoding the individual WT1 isoforms were compared to parental cells or control M1.Neo cells generated by transfection with a retroviral vector encoding neomycin-resistance. Morphological differentiation was enumerated by Leishman's staining of cytospin preparations and scoring the proportion of immature blast cells to cells at intermediate monocytic and mature macrophage stages of differentiation.