Loss of Adhesion of CD34 Cells to H120 Due to IL-3
| . | % Adherent Cells . | % Adherent Cells With IL-3 . | % Reduction in . |
|---|---|---|---|
| . | in Serum-Free Medium . | . | Adhesion With IL-3 . |
| CB | 92.1 | 24.8 | 73 |
| CB | 68.4 | 22.7 | 66.8 |
| CB | 83.7 | 30.4 | 63.6 |
| PB | 62.8 | 32.8 | 47.7 |
| PB | 65.4 | 41.6 | 36.4 |
| PB | 71.6 | 43.7 | 38.9 |
| . | % Adherent Cells . | % Adherent Cells With IL-3 . | % Reduction in . |
|---|---|---|---|
| . | in Serum-Free Medium . | . | Adhesion With IL-3 . |
| CB | 92.1 | 24.8 | 73 |
| CB | 68.4 | 22.7 | 66.8 |
| CB | 83.7 | 30.4 | 63.6 |
| PB | 62.8 | 32.8 | 47.7 |
| PB | 65.4 | 41.6 | 36.4 |
| PB | 71.6 | 43.7 | 38.9 |
CD34 cells isolated from individual CB, and PB samples were preincubated in the presence or absence of IL-3 10 ng/mL for 30 minutes before attaching to the H120 fragment. IL-3 resulted in consistent loss of adhesion (P < .001).
Abbreviations: CB, cord blood; PB, peripheral blood.