Fluorescence Intensity of CMCs Stained With Berberine Sulfate
| Cell Type . | Factors . | Fluorescence Intensity . |
|---|---|---|
| . | . | (rlu/cell, mean ± SE) . |
| +/+ CMCs | IL-33-150 | 1.03 ± 0.11 |
| IL-3 + NGF 5 ng/mL | 1.19 ± 0.12 | |
| IL-3 + NGF 50 ng/mL | 2.87 ± 0.203-151 | |
| IL-3 + NGF 500 ng/mL | 3.29 ± 0.133-151 | |
| mi/mi CMCs | IL-3 | 1.05 ± 0.11 |
| IL-3 + NGF 5 ng/mL | 0.99 ± 0.12 | |
| IL-3 + NGF 50 ng/mL | 1.42 ± 0.423-152 | |
| IL-3 + NGF 500 ng/mL | 1.06 ± 0.133-152 | |
| mi/mi CMCs overexpressing +-MITF | IL-3 | 1.01 ± 0.08 |
| IL-3 + NGF 5 ng/mL | 0.95 ± 0.10 | |
| IL-3 + NGF 50 ng/mL | 2.58 ± 0.14†ρ | |
| IL-3 + NGF 500 ng/mL | 3.21 ± 0.11†ρ | |
| mi/mi CMCs overexpressing mi-MITF | IL-3 | 1.09 ± 0.13 |
| IL-3 + NGF 5 ng/mL | 0.93 ± 0.11 | |
| IL-3 + NGF 50 ng/mL | 1.16 ± 0.123-152 | |
| IL-3 + NGF 500 ng/mL | 0.89 ± 0.123-152 |
| Cell Type . | Factors . | Fluorescence Intensity . |
|---|---|---|
| . | . | (rlu/cell, mean ± SE) . |
| +/+ CMCs | IL-33-150 | 1.03 ± 0.11 |
| IL-3 + NGF 5 ng/mL | 1.19 ± 0.12 | |
| IL-3 + NGF 50 ng/mL | 2.87 ± 0.203-151 | |
| IL-3 + NGF 500 ng/mL | 3.29 ± 0.133-151 | |
| mi/mi CMCs | IL-3 | 1.05 ± 0.11 |
| IL-3 + NGF 5 ng/mL | 0.99 ± 0.12 | |
| IL-3 + NGF 50 ng/mL | 1.42 ± 0.423-152 | |
| IL-3 + NGF 500 ng/mL | 1.06 ± 0.133-152 | |
| mi/mi CMCs overexpressing +-MITF | IL-3 | 1.01 ± 0.08 |
| IL-3 + NGF 5 ng/mL | 0.95 ± 0.10 | |
| IL-3 + NGF 50 ng/mL | 2.58 ± 0.14†ρ | |
| IL-3 + NGF 500 ng/mL | 3.21 ± 0.11†ρ | |
| mi/mi CMCs overexpressing mi-MITF | IL-3 | 1.09 ± 0.13 |
| IL-3 + NGF 5 ng/mL | 0.93 ± 0.11 | |
| IL-3 + NGF 50 ng/mL | 1.16 ± 0.123-152 | |
| IL-3 + NGF 500 ng/mL | 0.89 ± 0.123-152 |
CMCs were cultured in methylcellulose at a density of 104/mL; colonies were harvested 20 days after plating. The cells were stained with berberine sulfate, and fluorescence intensity of individual cells was examined using the system photometer. Each value represents the mean ± SE of 100 to 250 cells.
Abbreviation: rlu, relative lumen unit.
rmIL-3 was added at a concentration of 50 U/mL.
P < .01 by t-test v CMCs of the same type cultured with rmIL-3 alone.
P < .01 by t-test v +/+ CMCs in the same culture conditions.
ρ P < .01 by t-test v mi/mi CMCs in the same culture conditions.