Table 2. Normalization of the... | American Society of Hematology

Table 2.

Normalization of the Colony-Forming Potential of mi/mi CMCs by Overexpression of +-MITF in Medium Containing NGF

Days in Culture	Factors	No. of Colonies per 10⁴ CMCs^*
		+/+ CMCs	mi/mi CMCs	mi/mi CMCs Overexpressing +-MITF	mi/mi CMCs Overexpressing mi-MITF
14	IL-3^†	123 ± 8	127 ± 9	122 ± 7	120 ± 7
	IL-3 + NGF 50 ng/mL	206 ± 5^‡	113 ± 2	186 ± 7^‡	114 ± 2
	IL-3 + NGF 500 ng/mL	232 ± 4^‡	130 ± 2	235 ± 12^‡	137 ± 7
21	IL-3	135 ± 5	132 ± 2	133 ± 5	129 ± 3
	IL-3 + NGF 50 ng/mL	192 ± 3^‡	130 ± 1	167 ± 2^‡	124 ± 3
	IL-3 + NGF 500 ng/mL	231 ± 4^‡	127 ± 2	222 ± 6^‡	129 ± 2

Days in Culture	Factors	No. of Colonies per 10⁴ CMCs^*
		+/+ CMCs	mi/mi CMCs	mi/mi CMCs Overexpressing +-MITF	mi/mi CMCs Overexpressing mi-MITF
14	IL-3^†	123 ± 8	127 ± 9	122 ± 7	120 ± 7
	IL-3 + NGF 50 ng/mL	206 ± 5^‡	113 ± 2	186 ± 7^‡	114 ± 2
	IL-3 + NGF 500 ng/mL	232 ± 4^‡	130 ± 2	235 ± 12^‡	137 ± 7
21	IL-3	135 ± 5	132 ± 2	133 ± 5	129 ± 3
	IL-3 + NGF 50 ng/mL	192 ± 3^‡	130 ± 1	167 ± 2^‡	124 ± 3
	IL-3 + NGF 500 ng/mL	231 ± 4^‡	127 ± 2	222 ± 6^‡	129 ± 2

Mean ± SE of 5 experiments; 3 dishes were used for each experiment.

rmIL-3 was added at a concentration of 50 U/mL.

P < .01 by t-test v CMCs of the same type cultured with rmIL-3 alone.