Checkerboard Analysis of Lp(a)-Induced Monocyte Migration Across Polycarbonate Membranes
| Below Membrane . | Above Membrane . | |||
|---|---|---|---|---|
| . | . | . | . | . |
| . | . | |||
| . | . | . | . | . |
| . | HBSS . | Lp(a) 33 . | Lp(a) 100 (μg/mL) . | Lp(a) 300 . |
| HBSS | 5.0 ± 0.6 | 8.3 ± 0.6 | 7.6 ± 0.2 | 7.6 ± 0.9 |
| Lp(a) (μg/mL) | ||||
| 33 | 10.4 ± 1.1 | 8.3 ± 0.6 | 8.3 ± 0.5 | 7.7 ± 0.3 |
| 100 | 15.0 ± 0.8* | 12.8 ± 0.8† | 9.6 ± 0.5 | 7.2 ± 0.5 |
| 300 | 23.9 ± 1.1* | 20.8 ± 0.9* | 16.0 ± 1.1* | 9.6 ± 2.2 |
| Below Membrane . | Above Membrane . | |||
|---|---|---|---|---|
| . | . | . | . | . |
| . | . | |||
| . | . | . | . | . |
| . | HBSS . | Lp(a) 33 . | Lp(a) 100 (μg/mL) . | Lp(a) 300 . |
| HBSS | 5.0 ± 0.6 | 8.3 ± 0.6 | 7.6 ± 0.2 | 7.6 ± 0.9 |
| Lp(a) (μg/mL) | ||||
| 33 | 10.4 ± 1.1 | 8.3 ± 0.6 | 8.3 ± 0.5 | 7.7 ± 0.3 |
| 100 | 15.0 ± 0.8* | 12.8 ± 0.8† | 9.6 ± 0.5 | 7.2 ± 0.5 |
| 300 | 23.9 ± 1.1* | 20.8 ± 0.9* | 16.0 ± 1.1* | 9.6 ± 2.2 |
Monocytes were permitted to migrate for 90 minutes across polycarbonate membranes with a pore size of 5 μm. Different concentrations of Lp(a) were placed in the upper and/or lower compartment of the Transwell chambers. Cell numbers represent the mean ± SEM of migrated monocytes in seven oil immersion fields counted in triplicates from four experiments.
P < .01 and † P < .05 by Newman-Keuls test versus corresponding values with HBSS in the lower compartment of the chemotaxis chamber.