Effects of Neutralizing Antibodies on Monocyte Migration Elicited by MCP-1, MIP-1α, C5a, and Lp(a)
| . | No. of Migrated Cells . | n‡ . |
|---|---|---|
| Control | 4.8 ± 0.5 | 5 |
| MCP-1 (50 ng/mL) | 21.9 ± 1.8* | 4 |
| + anti–MCP-1 (10 μg/mL) | 6.1 ± 0.2† | 4 |
| MIP-1α (50 ng/mL) | 15.3 ± 1.2* | 4 |
| + anti–MIP-1α (10 μg/mL) | 5.7 ± 0.3† | 4 |
| C5a (1.0 nmol/L) | 22.0 ± 0.7* | 4 |
| + anti-C5a (80 μg/mL) | 5.2 ± 0.7† | 4 |
| Lp(a) (300 μg/mL) | 19.7 ± 0.8* | 5 |
| + anti–MCP-1 (10 μg/mL) | 19.4 ± 0.3 | 4 |
| + anti–MIP-1α (10 μg/mL) | 20.9 ± 1.3 | 4 |
| + anti-C5a (80 μg/mL) | 20.8 ± 0.9 | 4 |
| . | No. of Migrated Cells . | n‡ . |
|---|---|---|
| Control | 4.8 ± 0.5 | 5 |
| MCP-1 (50 ng/mL) | 21.9 ± 1.8* | 4 |
| + anti–MCP-1 (10 μg/mL) | 6.1 ± 0.2† | 4 |
| MIP-1α (50 ng/mL) | 15.3 ± 1.2* | 4 |
| + anti–MIP-1α (10 μg/mL) | 5.7 ± 0.3† | 4 |
| C5a (1.0 nmol/L) | 22.0 ± 0.7* | 4 |
| + anti-C5a (80 μg/mL) | 5.2 ± 0.7† | 4 |
| Lp(a) (300 μg/mL) | 19.7 ± 0.8* | 5 |
| + anti–MCP-1 (10 μg/mL) | 19.4 ± 0.3 | 4 |
| + anti–MIP-1α (10 μg/mL) | 20.9 ± 1.3 | 4 |
| + anti-C5a (80 μg/mL) | 20.8 ± 0.9 | 4 |
Monocytes were permitted to migrate across polycarbonate membranes with a pore size of 5 μm for 90 minutes. Results represent the mean ± SEM of migrated cells in seven oil-immersion fields.
P < .01 versus HBSS control.
P < .01 versus positive control without neutralizing antibodies.
The number of independent experiments is given as n.