WCP, FISH, and p53 Analysis in 17 MDS and AML Patients With 17p Deletion
| Patient No. . | WCP . | FISH Analysis . | P53 Analysis . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| D17Z1 Centromere 17 . | 961F10 17p11.2 D17S922 D17S921 D17S839 D17S955 . | 904B5 17p11.2 D17S799 D17S936 . | 914C7 17p12 D17S954 . | p53 17p13.1 . | MD 17p13.3 D17S379 . | p53 Mutation (SSCP) . | p53 Overexpression (immunocytochemistry) . | ||
| t(5;17) translocation | |||||||||
| 1 | der(5)t(5;17) | − | − | − | − | − | − | ND | ND |
| 2 | der(17)t(5;17) | + | + | + | − | − | − | Exon 5 | ND |
| 3 | der(5)t(5;17) | + | + | − | − | − | − | ND | Yes |
| 4 | der(5)t(5;17) | − | − | − | − | − | − | Exon 7 | Yes |
| 5 | der(17)t(5;17) | + | − | − | − | − | − | No | No |
| 6* | der(17)t(5;17) | + | − | − | − | − | − | Exon 5 | Yes |
| der(18)t(17;18) | − | ND | ND | ND | ND | ND | |||
| 7 | der(?)t(5;17) | ND | ND | ND | ND | ND | ND | ND | ND |
| 8 | der(5)t(5.17) | − | − | − | − | − | − | No | No |
| mar(7;17) | + | + | + | + | + | + | |||
| 9 | der(5)t(5;17) | − | − | − | − | − | − | ND | ND |
| r(17) | + | + | + | + | + | + | |||
| Rearrangement between 17p and another chromosome | |||||||||
| 10 | der(17)t(12;17) | + | + | + | − | − | − | Exon 8 | Yes |
| 11 | dic(12;17) | + | + | + | + | − | − | Exon 8 | Yes |
| 12 | der(17)t(17;21) | + | + | + | + | − | − | ND | Yes |
| 13 | der(17)t(17;22) | + | + | + | + | + | − | Exon 7 | Yes |
| −17 | − | − | − | − | − | − | |||
| 14 | add(17) | + | + | + | + | − | − | ND | Yes |
| 15 | add(17) | − | − | − | − | − | − | Exon 7 | ND |
| Monosomy 17 and i(17q) | |||||||||
| 16 | −17 | − | − | − | − | − | − | Exon 5 | Yes |
| 17 | idic(17)(p11) | ++ | − | − | − | − | − | ND | ND |
| Patient No. . | WCP . | FISH Analysis . | P53 Analysis . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| D17Z1 Centromere 17 . | 961F10 17p11.2 D17S922 D17S921 D17S839 D17S955 . | 904B5 17p11.2 D17S799 D17S936 . | 914C7 17p12 D17S954 . | p53 17p13.1 . | MD 17p13.3 D17S379 . | p53 Mutation (SSCP) . | p53 Overexpression (immunocytochemistry) . | ||
| t(5;17) translocation | |||||||||
| 1 | der(5)t(5;17) | − | − | − | − | − | − | ND | ND |
| 2 | der(17)t(5;17) | + | + | + | − | − | − | Exon 5 | ND |
| 3 | der(5)t(5;17) | + | + | − | − | − | − | ND | Yes |
| 4 | der(5)t(5;17) | − | − | − | − | − | − | Exon 7 | Yes |
| 5 | der(17)t(5;17) | + | − | − | − | − | − | No | No |
| 6* | der(17)t(5;17) | + | − | − | − | − | − | Exon 5 | Yes |
| der(18)t(17;18) | − | ND | ND | ND | ND | ND | |||
| 7 | der(?)t(5;17) | ND | ND | ND | ND | ND | ND | ND | ND |
| 8 | der(5)t(5.17) | − | − | − | − | − | − | No | No |
| mar(7;17) | + | + | + | + | + | + | |||
| 9 | der(5)t(5;17) | − | − | − | − | − | − | ND | ND |
| r(17) | + | + | + | + | + | + | |||
| Rearrangement between 17p and another chromosome | |||||||||
| 10 | der(17)t(12;17) | + | + | + | − | − | − | Exon 8 | Yes |
| 11 | dic(12;17) | + | + | + | + | − | − | Exon 8 | Yes |
| 12 | der(17)t(17;21) | + | + | + | + | − | − | ND | Yes |
| 13 | der(17)t(17;22) | + | + | + | + | + | − | Exon 7 | Yes |
| −17 | − | − | − | − | − | − | |||
| 14 | add(17) | + | + | + | + | − | − | ND | Yes |
| 15 | add(17) | − | − | − | − | − | − | Exon 7 | ND |
| Monosomy 17 and i(17q) | |||||||||
| 16 | −17 | − | − | − | − | − | − | Exon 5 | Yes |
| 17 | idic(17)(p11) | ++ | − | − | − | − | − | ND | ND |
*In patient no. 6, no t(17;18) cells could be analyzed by FISH.
Abbreviation: ND, not done.