Partitioning of Endogenous and Exogenous GPI-Anchored Proteins in Triton X-100–Insoluble Fractions of Erythrocyte Ghost Membranes
| Protein . | Enrichment (Relative to Bulk Protein)* . | n . |
|---|---|---|
| Endogenous AChE | 1.69 ± 0.10 | 9 |
| Endogenous AChE (37°C) | 0.016 ± 0.003 | 9 |
| Endogenous CD59 | 1.55 ± 0.05 | 2 |
| Endogenous CD59 (37°C) | 0.019 ± 0.012 | 2 |
| Exogenous AChE | 0.59 ± 0.04 | 3 |
| VSG | 0.88 ± 0.06 | 8 |
| VSG GPI anchor | 0.20 | 1 |
| Procyclin | 0.90 ± 0.09 | 3 |
| Procyclin GPI anchor | 0.20 ± 0.05 | 3 |
| Procyclin after incubation-151 | 0.94 | 1 |
| Procyclin GPI anchor after incubation-151 | 0.20 | 1 |
| Protein . | Enrichment (Relative to Bulk Protein)* . | n . |
|---|---|---|
| Endogenous AChE | 1.69 ± 0.10 | 9 |
| Endogenous AChE (37°C) | 0.016 ± 0.003 | 9 |
| Endogenous CD59 | 1.55 ± 0.05 | 2 |
| Endogenous CD59 (37°C) | 0.019 ± 0.012 | 2 |
| Exogenous AChE | 0.59 ± 0.04 | 3 |
| VSG | 0.88 ± 0.06 | 8 |
| VSG GPI anchor | 0.20 | 1 |
| Procyclin | 0.90 ± 0.09 | 3 |
| Procyclin GPI anchor | 0.20 ± 0.05 | 3 |
| Procyclin after incubation-151 | 0.94 | 1 |
| Procyclin GPI anchor after incubation-151 | 0.20 | 1 |
*The numbers represent mean values ± SD from n independent experiments. Except where indicated, the extractions were carried out at 4°C.
Detergent extraction was performed after incubation of erythrocytes for 24 hours at 37°C.