Cloning of ELM Cells on NormalSl/Sl Stroma
| . | % Cloning Efficiency . |
|---|---|
| Cells transferred from MS5 stroma to: | |
| MS5 | 36.5 |
| MS5 + SCF | 44.5 |
| Sl/Sl stroma | 0.1 |
| Sl/Sl stroma + SCF | 49 |
| SCF | 32.5 |
| No growth factors | 2 |
| Cells transferred from Sl/Sl stroma to: | |
| MS5 | 30.5 |
| Sl/Sl stroma | 41 |
| Sl/Sl stroma + SCF | 54 |
| SCF | 0.4 |
| No growth factors | 5.5 |
| . | % Cloning Efficiency . |
|---|---|
| Cells transferred from MS5 stroma to: | |
| MS5 | 36.5 |
| MS5 + SCF | 44.5 |
| Sl/Sl stroma | 0.1 |
| Sl/Sl stroma + SCF | 49 |
| SCF | 32.5 |
| No growth factors | 2 |
| Cells transferred from Sl/Sl stroma to: | |
| MS5 | 30.5 |
| Sl/Sl stroma | 41 |
| Sl/Sl stroma + SCF | 54 |
| SCF | 0.4 |
| No growth factors | 5.5 |
ELM cells grown on MS5 stroma were cloned on MS5 cells or SCF-deficient Sl/Sl stroma (UNC) either in the presence or absence of 10 ng/mL SCF. ELM cells grown on Sl/Sl stroma were cloned in SCF, IGF-1, or no growth factors.