Expression of γIFN and HLA-DR in AIDS-KS, C-KS Lesions, and Uninvolved Tissues and Correlation With the Presence of HHV-8 and EBV
| Specimen . | Average % (range) γIFN . | Positive Cells HLA-DR* . | PCR . | |
|---|---|---|---|---|
| HHV-8 . | EBV . | |||
| AIDS-KS | ||||
| 1 skin | 45 (41-49) | 73 (70-75) | ND | ND |
| 2 skin | 32 (27-36) | 50 (48-52) | + | + |
| 3 skin | 11 (10-11) | 62 (58-65) | + | − |
| 4 skin | 42 (33-50) | 80 (75-84) | + | − |
| 5 skin | 50 (34-50) | 80 (80-81) | + | − |
| 6 skin | 30 (28-32) | 55 (49-61) | + | − |
| 7 skin | 28 (26-30) | 59 (55-62) | + | − |
| 8 skin | 43 (41-44) | 47 (36-65) | + | + |
| 9 skin | 29 (19-39) | 45 (43-64) | + | − |
| 10 skin | 12 (7-17) | 45 (43-64) | + | − |
| 11 skin | 15 (11-21) | ND | − | − |
| 12 skin | 39 (38-40) | 35 (29-41) | − | − |
| 13 skin | 9 (3-15) | ND | + | − |
| 14 skin | 45 (40-50) | 64 (58-70) | + | + |
| 15 skin | 40 (40-40) | ND | + | ND |
| 16 skin | 36 (25-49) | 44 (38-50) | + | ND |
| 17 skin | 35 (29-41) | 47 (38-56) | + | ND |
| 18 skin | 28 (13-49) | ND | + | ND |
| 19 lymphnode | 40 (39-40) | 69 (66-72) | + | + |
| Classical KS | ||||
| 1 skin | 43 (41-60) | 30 (30-31) | − | + |
| 2 skin | 26 (25-27) | 19 (17-21) | + | − |
| 3 skin | 5 (5-7) | ND | + | ND |
| Uninvolved | ||||
| 1 skin | 2 (2-2) | 23 (21-25) | − | ND |
| 2 skin | 5 (5-5) | 19 (19) | − | ND |
| 3 skin | 10 (10-11) | 32 (28-37) | + | ND |
| 4 skin | 12 (7-17) | 41 (32-51) | + | ND |
| 5 skin | 0 | 11 (11-11) | − | ND |
| Specimen . | Average % (range) γIFN . | Positive Cells HLA-DR* . | PCR . | |
|---|---|---|---|---|
| HHV-8 . | EBV . | |||
| AIDS-KS | ||||
| 1 skin | 45 (41-49) | 73 (70-75) | ND | ND |
| 2 skin | 32 (27-36) | 50 (48-52) | + | + |
| 3 skin | 11 (10-11) | 62 (58-65) | + | − |
| 4 skin | 42 (33-50) | 80 (75-84) | + | − |
| 5 skin | 50 (34-50) | 80 (80-81) | + | − |
| 6 skin | 30 (28-32) | 55 (49-61) | + | − |
| 7 skin | 28 (26-30) | 59 (55-62) | + | − |
| 8 skin | 43 (41-44) | 47 (36-65) | + | + |
| 9 skin | 29 (19-39) | 45 (43-64) | + | − |
| 10 skin | 12 (7-17) | 45 (43-64) | + | − |
| 11 skin | 15 (11-21) | ND | − | − |
| 12 skin | 39 (38-40) | 35 (29-41) | − | − |
| 13 skin | 9 (3-15) | ND | + | − |
| 14 skin | 45 (40-50) | 64 (58-70) | + | + |
| 15 skin | 40 (40-40) | ND | + | ND |
| 16 skin | 36 (25-49) | 44 (38-50) | + | ND |
| 17 skin | 35 (29-41) | 47 (38-56) | + | ND |
| 18 skin | 28 (13-49) | ND | + | ND |
| 19 lymphnode | 40 (39-40) | 69 (66-72) | + | + |
| Classical KS | ||||
| 1 skin | 43 (41-60) | 30 (30-31) | − | + |
| 2 skin | 26 (25-27) | 19 (17-21) | + | − |
| 3 skin | 5 (5-7) | ND | + | ND |
| Uninvolved | ||||
| 1 skin | 2 (2-2) | 23 (21-25) | − | ND |
| 2 skin | 5 (5-5) | 19 (19) | − | ND |
| 3 skin | 10 (10-11) | 32 (28-37) | + | ND |
| 4 skin | 12 (7-17) | 41 (32-51) | + | ND |
| 5 skin | 0 | 11 (11-11) | − | ND |
Frozen sections from involved or uninvolved skin and a lymph node of AIDS-KS and C-KS patients were stained by the APAAP method using MoAbs directed against γIFN and HLA-DR. Reported are the average percent of positive cells from 5 HPMF (100× magnification) per slide and in parenthesis is shown the range of positivity from different areas of the lesions. In KS tissues γIFN positive cells were of mononuclear and spindle-shaped morphology. In control tissues γIFN positivity was in mononuclear cells. In KS tissues HLA-DR was strongly expressed in vessels, spindle cells and mononuclear cells. A total of 1 to 10 μg of tissue DNA was analyzed by PCR for HHV-8 and EBV DNA sequences. Each case was tested at least twice. Negative cases resulted negative also by using 10 μg DNA and different tissue sections. HHV-8 negative cases were consistently negative independently from the amount of DNA used and were early KS lesions. All the PCR negative samples were positive for β-globin amplification. PCR analysis was also performed for HHV-6, HHV-7, and CMV. These herpesviruses resulted absent or present at a low frequency (HHV-6 [5%] or CMV [8%]) in association with HHV-8.
Abbreviation: ND, not done.
Positivity for HLA-DR was also present in vessels.