γIFN-Treated or TCM-Treated HUVE Cells Induce in Nude Mice Vascular Lesions Closely Resembling KS That Are Increased by the HIV-1 Tat Protein
| Cells . | Treatment . | Lesion* . | Angiogenesis† . | Spindle Cells† . | Edema† . |
|---|---|---|---|---|---|
| HUVE | — | 0% (12) | 0% (0) | 0% (0) | 0% (0) |
| HUVE | TCM | 59% (17) | 100% (5) | 100% (6) | 100% (5) |
| HUVE | γIFN | 41% (17) | 70% (2) | 82% (3) | 82% (4) |
| HUVE | TCM + Tat | 100% (10) | 100% (8) | 100% (8) | 100% (5) |
| HUVE | γIFN + Tat | 60% (15) | 73% (3) | 100% (4) | 100% (5) |
| Cells . | Treatment . | Lesion* . | Angiogenesis† . | Spindle Cells† . | Edema† . |
|---|---|---|---|---|---|
| HUVE | — | 0% (12) | 0% (0) | 0% (0) | 0% (0) |
| HUVE | TCM | 59% (17) | 100% (5) | 100% (6) | 100% (5) |
| HUVE | γIFN | 41% (17) | 70% (2) | 82% (3) | 82% (4) |
| HUVE | TCM + Tat | 100% (10) | 100% (8) | 100% (8) | 100% (5) |
| HUVE | γIFN + Tat | 60% (15) | 73% (3) | 100% (4) | 100% (5) |
Reported is the percentage of mice developing macroscopic vascular lesions and the percentage of mice developing histologic alterations. HUVE cells were treated with γIFN (102 U/mL) or TCM (1:4) and 3 × 106 cells were inoculated in nude mice in the presence or in the absence of Tat (10 μg) as described in Materials and Methods. Mice were killed 6 to 7 days after inoculation and tissue slides examined after H & E staining and graded as described previously.32 Inoculation of Tat alone did not induce lesions or histologic alterations, as described previously.32
Percentage of mice developing macroscopic vascular lesions (size ranging from 4 × 5 to 7 × 7 mm). Parenthesis show the number of inoculated mice.
Percentage of mice developing histologic alterations. Parenthesis show the average “intensity value” for each histopathologic feature observed per each experimental condition.