Bone Marrow Analysis
| Nucleated Cell Types . | Wild-type . | IL-6 (−/−) . | G-CSFR (−/−) . | IL-6 × GR (−/−) . |
|---|---|---|---|---|
| . | (% ± SD) . | (% ± SD) . | (% ± SD) . | (% ± SD) . |
| Total nucleated cells (×10−6) (per femur) | 22.6 ± 4.0 | 20.8 ± 2.7 | 25.1 ± 8.4 | 21.3 ± 3.7 |
| Myeloblasts | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.3 ± 0.2 | 0.2 ± 0.2 |
| Promyelocytes | 1.4 ± 0.6 | 1.6 ± 0.5 | 0.8 ± 0.2 | 1.0 ± 0.5 |
| Myelocytes | 3.2 ± 1.0 | 3.8 ± 1.5 | 2.2 ± 0.9 | 2.2 ± 0.9 |
| Metamyelocytes neutrophil | 17.2 ± 4.7 | 16.7 ± 3.7 | 10.2 ± 4.7* | 9.0 ± 3.2† |
| Band and segmented neutrophil | 26.4 ± 8.0 | 24.2 ± 6.4 | 9.2 ± 2.6‡ | 5.5 ± 1.2‡ρ |
| Eosinophil lineage | 3.7 ± 2.5 | 3.2 ± 2.0 | 2.1 ± 1.2 | 5.2 ± 3.9 |
| Lymphocyte lineage | 23.1 ± 7.8 | 23.8 ± 2.5 | 44.8 ± 5.1‡ | 40.4 ± 5.8† |
| Normoblasts | 24.7 ± 8.0 | 26.3 ± 10.1 | 30.2 ± 6.6 | 36.9 ± 7.7* |
| Myeloid/erythroid ratio | 2.4 ± 1.8 | 2.2 ± 1.4 | 0.8 ± 0.3 | 0.6 ± 0.2* |
| Nucleated Cell Types . | Wild-type . | IL-6 (−/−) . | G-CSFR (−/−) . | IL-6 × GR (−/−) . |
|---|---|---|---|---|
| . | (% ± SD) . | (% ± SD) . | (% ± SD) . | (% ± SD) . |
| Total nucleated cells (×10−6) (per femur) | 22.6 ± 4.0 | 20.8 ± 2.7 | 25.1 ± 8.4 | 21.3 ± 3.7 |
| Myeloblasts | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.3 ± 0.2 | 0.2 ± 0.2 |
| Promyelocytes | 1.4 ± 0.6 | 1.6 ± 0.5 | 0.8 ± 0.2 | 1.0 ± 0.5 |
| Myelocytes | 3.2 ± 1.0 | 3.8 ± 1.5 | 2.2 ± 0.9 | 2.2 ± 0.9 |
| Metamyelocytes neutrophil | 17.2 ± 4.7 | 16.7 ± 3.7 | 10.2 ± 4.7* | 9.0 ± 3.2† |
| Band and segmented neutrophil | 26.4 ± 8.0 | 24.2 ± 6.4 | 9.2 ± 2.6‡ | 5.5 ± 1.2‡ρ |
| Eosinophil lineage | 3.7 ± 2.5 | 3.2 ± 2.0 | 2.1 ± 1.2 | 5.2 ± 3.9 |
| Lymphocyte lineage | 23.1 ± 7.8 | 23.8 ± 2.5 | 44.8 ± 5.1‡ | 40.4 ± 5.8† |
| Normoblasts | 24.7 ± 8.0 | 26.3 ± 10.1 | 30.2 ± 6.6 | 36.9 ± 7.7* |
| Myeloid/erythroid ratio | 2.4 ± 1.8 | 2.2 ± 1.4 | 0.8 ± 0.3 | 0.6 ± 0.2* |
Cell counts and 500-count manual leukocyte-differentials were performed on mononuclear cells recovered from the femurs of 6 to 7 sex-matched mice of each genotype. Data represent the mean ± SD.
P < .05 compared with wild-type mice.
P < .01 compared with wild-type mice.
P < .001 compared with wild-type mice.
ρ P < .01 compared with G-CSFR (−/−) mice.