Blast Colony Formation per 106 AML PB Cells With Different Cytokine Combinations
| Patient . | Growth Conditions5-150 . | |||
|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . |
| 1 | 70 | 125 | 10 | 0 |
| 2 | 10 | 30 | 0 | 0 |
| 3 | 1,980 | 2,350 | 1,750 | 0 |
| 4 | 320 | 740 | 1,010 | 10 |
| 5 | >10,000 | 16,500 | >10,000 | 65 |
| 6 | 215 | 240 | 180 | 155 |
| 7 | 24,250 | >24,250 | 4,220 | 2,900 |
| 8 | 15 | 45 | 5 | 10 |
| 9 | 2,050 | 2,625 | 800 | 250 |
| 10 | 170 | 215 | 20 | 0 |
| Patient . | Growth Conditions5-150 . | |||
|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . |
| 1 | 70 | 125 | 10 | 0 |
| 2 | 10 | 30 | 0 | 0 |
| 3 | 1,980 | 2,350 | 1,750 | 0 |
| 4 | 320 | 740 | 1,010 | 10 |
| 5 | >10,000 | 16,500 | >10,000 | 65 |
| 6 | 215 | 240 | 180 | 155 |
| 7 | 24,250 | >24,250 | 4,220 | 2,900 |
| 8 | 15 | 45 | 5 | 10 |
| 9 | 2,050 | 2,625 | 800 | 250 |
| 10 | 170 | 215 | 20 | 0 |
Light density PB cells from patients with newly diagnosed AML were plated in methylcellulose assay containing 30% FCS and human cytokine(s) as: (1) SF (50 ng/mL) + IL-3 (10 ng/mL) + GM-CSF (10 ng/mL), (2) SF (50 ng/mL) + IL-3 (10 ng/mL) + GM-CSF (10 ng/mL) + FL (50 ng/mL), (3) FL (50 ng/mL), and (4) No added cytokines.