Frequency and Proliferative Capacity of LTC-IC in AML PB
| Patient . | Day 0 CFC/106 Cells . | Feeder7-150 . | LTC-IC/106 Cells . | CFC/LTC-IC7-151 . |
|---|---|---|---|---|
| . | (% abnormal) . | . | (% abnormal) . | . |
| 3 | 3,150 (90) | HMF | 15 (32) | 1.4 |
| 4 | 485 (73) | HMF | 50 (64) | 1.8 |
| 7 | 31,260 (95) | HMF | 121 (55) | 1.2 |
| Mixed | 116 (73) | 2.7 | ||
| 8 | 45 (67) | HMF | 4 (7) | 4.4 |
| Mixed | 4 (9) | 12.8 | ||
| 9 | 1,138 (0) | HMF | 40 (0) | 1.7 |
| 10 | 225 (50) | HMF | 3 (21) | 2.1 |
| Patient . | Day 0 CFC/106 Cells . | Feeder7-150 . | LTC-IC/106 Cells . | CFC/LTC-IC7-151 . |
|---|---|---|---|---|
| . | (% abnormal) . | . | (% abnormal) . | . |
| 3 | 3,150 (90) | HMF | 15 (32) | 1.4 |
| 4 | 485 (73) | HMF | 50 (64) | 1.8 |
| 7 | 31,260 (95) | HMF | 121 (55) | 1.2 |
| Mixed | 116 (73) | 2.7 | ||
| 8 | 45 (67) | HMF | 4 (7) | 4.4 |
| Mixed | 4 (9) | 12.8 | ||
| 9 | 1,138 (0) | HMF | 40 (0) | 1.7 |
| 10 | 225 (50) | HMF | 3 (21) | 2.1 |
Frequency was determined by limiting dilution analysis as described in Materials and Methods and in Sutherland et al.10
Feeders as described in Table 2.
CFC per LTC-IC were calculated as average values by dividing the total number of CFC produced in all of the limiting dilution cultures plated by the number of calculated LTC-IC used to initiate those cultures for each culture condition tested.