CFC in Week 5 Long-Term Cultures of AML Samples Under Various Growth Conditions
| Patient . | Tissue . | CFC per 106 Cells . | Fold Change From HMF (% abnormal*) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | (% abnormal*) . | Sl/Sl . | Sl/Sl JIL3 + SF . | Sl/Sl + FL . | Sl/Sl JIL3 + FL . | Mixed . | Mixed + FL . | |
| . | . | Day 0 . | HMF . | . | . | . | . | . | . |
| . | . | . | 5 wk . | . | . | . | . | . | . |
| 1 | BM | 165 (40) | 53 (25) | 2 (6) | 3 (7)6-152 | ||||
| 2 | BM | 30 (33) | 9 (25) | 2 (0)6-152 | 6 (21) | ||||
| 3 | BM | ND | 13 (53) | ND | 8 (34) | ||||
| 3 | PB | 3,150 (90) | 15 (32) | 5 (60) | 4 (68) | 30 (5)6-152 | 12 (0)6-152 | ||
| 46-151 | PB | 485 (73) | 34 (88) | 0.4 (85) | 2 (87) | 1 (11)6-152 | 2 (23)6-152 | ||
| 55 (71) | 1 (43) | 1 (52) | 0.8 (3)6-152 | 0.7 (15)6-152 | |||||
| 5 | PB | 48,100 (100) | 307 (100) | 0.02 (92) | 0.8 (100) | 2 (100) | 0.6 (88) | ||
| 6 | PB | 158 (87) | 2,790 (80) | 0.7 (82) | 0.5 (69) | 0.6 (82) | 0.4 (82) | ||
| 7 | PB | 31,260 (95) | 129 (0) | 0.03 (29) | 0.7 (60) | 0.4 (68) | 1 (78) | 0.3 (52) | 4 (50) |
| 8 | PB | 45 (67) | 6 (65) | 0.5 (44) | 3 (33) | 3 (35) | 16 (29)6-152 | 3 (28)6-152 | 59 (38) |
| 10 | PB | 225 (50) | 3 (21) | 2 (0) | 25 (4) | 185 (13) | 547 (0)6-152 | 11 (0)6-152 | 429 (8) |
| Patient . | Tissue . | CFC per 106 Cells . | Fold Change From HMF (% abnormal*) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | (% abnormal*) . | Sl/Sl . | Sl/Sl JIL3 + SF . | Sl/Sl + FL . | Sl/Sl JIL3 + FL . | Mixed . | Mixed + FL . | |
| . | . | Day 0 . | HMF . | . | . | . | . | . | . |
| . | . | . | 5 wk . | . | . | . | . | . | . |
| 1 | BM | 165 (40) | 53 (25) | 2 (6) | 3 (7)6-152 | ||||
| 2 | BM | 30 (33) | 9 (25) | 2 (0)6-152 | 6 (21) | ||||
| 3 | BM | ND | 13 (53) | ND | 8 (34) | ||||
| 3 | PB | 3,150 (90) | 15 (32) | 5 (60) | 4 (68) | 30 (5)6-152 | 12 (0)6-152 | ||
| 46-151 | PB | 485 (73) | 34 (88) | 0.4 (85) | 2 (87) | 1 (11)6-152 | 2 (23)6-152 | ||
| 55 (71) | 1 (43) | 1 (52) | 0.8 (3)6-152 | 0.7 (15)6-152 | |||||
| 5 | PB | 48,100 (100) | 307 (100) | 0.02 (92) | 0.8 (100) | 2 (100) | 0.6 (88) | ||
| 6 | PB | 158 (87) | 2,790 (80) | 0.7 (82) | 0.5 (69) | 0.6 (82) | 0.4 (82) | ||
| 7 | PB | 31,260 (95) | 129 (0) | 0.03 (29) | 0.7 (60) | 0.4 (68) | 1 (78) | 0.3 (52) | 4 (50) |
| 8 | PB | 45 (67) | 6 (65) | 0.5 (44) | 3 (33) | 3 (35) | 16 (29)6-152 | 3 (28)6-152 | 59 (38) |
| 10 | PB | 225 (50) | 3 (21) | 2 (0) | 25 (4) | 185 (13) | 547 (0)6-152 | 11 (0)6-152 | 429 (8) |
Growth conditions as shown on Table 2.
% Abnormal, % cytogenetically abnormal colonies detected by FISH.
Data for two separate experiments in LTC.
The difference in the proportion of cytogenetically abnormal CFC between this LTC condition and that of LTC containing HMF achieved a P value <.05 by chi-square analysis.