Effect of Ectopic HOX-B8 Expression on 32D Cells Treated With GM-CSF
| Clone . | Treatment3-150 . | Cell Type (%)3-150 . | ||||
|---|---|---|---|---|---|---|
| . | . | Blast . | Granulocytes . | Macrophage . | NSE Pos (%)3-150 . | |
| . | . | . | Inter . | Inter . | Mat . | . |
| 32Dcl3 | IL-3 | 92 | 8 | 0 | 0 | 1 |
| GM-CSF | 84 | 16 | 0 | 0 | 3 | |
| 32Dneo | IL-3 | 89 | 11 | 0 | 0 | 1 |
| GM-CSF | 87 | 13 | 0 | 0 | 6 | |
| 32DHOX-B8/6 | IL-3 | 88 | 12 | 0 | 0 | 30 |
| GM-CSF | 4 | 0 | 30 | 66 | 95 | |
| 32DHOX-B8/9 | IL-3 | 86 | 14 | 0 | 0 | 8 |
| GM-CSF | 14 | 0 | 32 | 54 | 95 | |
| 32DHOX-B8/18 | IL-3 | 85 | 15 | 0 | 0 | 36 |
| GM-CSF | 5 | 0 | 31 | 64 | 97 | |
| Clone . | Treatment3-150 . | Cell Type (%)3-150 . | ||||
|---|---|---|---|---|---|---|
| . | . | Blast . | Granulocytes . | Macrophage . | NSE Pos (%)3-150 . | |
| . | . | . | Inter . | Inter . | Mat . | . |
| 32Dcl3 | IL-3 | 92 | 8 | 0 | 0 | 1 |
| GM-CSF | 84 | 16 | 0 | 0 | 3 | |
| 32Dneo | IL-3 | 89 | 11 | 0 | 0 | 1 |
| GM-CSF | 87 | 13 | 0 | 0 | 6 | |
| 32DHOX-B8/6 | IL-3 | 88 | 12 | 0 | 0 | 30 |
| GM-CSF | 4 | 0 | 30 | 66 | 95 | |
| 32DHOX-B8/9 | IL-3 | 86 | 14 | 0 | 0 | 8 |
| GM-CSF | 14 | 0 | 32 | 54 | 95 | |
| 32DHOX-B8/18 | IL-3 | 85 | 15 | 0 | 0 | 36 |
| GM-CSF | 5 | 0 | 31 | 64 | 97 | |
Cells were seeded as indicated in Materials and Methods. Cell morphology and NSE staining were determined after 4 days with IL-3 (10% WEHI-3 CM) or 10 days with GM-CSF (10 ng/mL).