Table 2. Effect of Ectopic HOX-B8... | American Society of Hematology

Table 2.

Effect of Ectopic HOX-B8 Expression on HL-60 Induced to Undergo Monocytic or Granulocytic Differentiation

Clone	Inducer^*	Cell Type (%)^†			Positive (%)^†		Cell No.^†
		Blast	Inter	Mat	NSE	NBT	10⁶/mL
HL-60	None	100	0	0	1	1	2.53
	PMA	0	13	87	90	0	0.35
	DMSO	0	4	96	0	76	1.32
HL-60neo5	None	100	0	0	1	1	2.41
	PMA	0	16	84	92	0	0.36
	DMSO	0	8	92	0	71	1.25
HL-60HOX-B8/2	None	100	0	0	1	1	2.64
	PMA	0	15	85	95	0	0.32
	DMSO	2	79	19	0	55	1.27
HL-60HOX-B8/7	None	100	0	0	1	1	2.71
	PMA	0	14	86	92	0	0.38
	DMSO	8	82	10	0	53	1.33

Clone	Inducer^*	Cell Type (%)^†			Positive (%)^†		Cell No.^†
		Blast	Inter	Mat	NSE	NBT	10⁶/mL
HL-60	None	100	0	0	1	1	2.53
	PMA	0	13	87	90	0	0.35
	DMSO	0	4	96	0	76	1.32
HL-60neo5	None	100	0	0	1	1	2.41
	PMA	0	16	84	92	0	0.36
	DMSO	0	8	92	0	71	1.25
HL-60HOX-B8/2	None	100	0	0	1	1	2.64
	PMA	0	15	85	95	0	0.32
	DMSO	2	79	19	0	55	1.27
HL-60HOX-B8/7	None	100	0	0	1	1	2.71
	PMA	0	14	86	92	0	0.38
	DMSO	8	82	10	0	53	1.33

Cells were seeded at 0.2 × 10⁶ cells/mL with PMA (5 nmol/L), or at 0.4 × 10⁶/mL with DMSO (1.3%). Two HL-60neo and four HL-60HOX-B8 cell lines were analyzed, and data from representative clones is shown.

†

Cell number and morphology were determined after 3 days for untreated cells and cells treated with PMA and after 5 days for cells treated with DMSO. NSE and NBT reduction were determined after 5 days in culture, and positive cells contain red (NSE) or blue (NBT) granules in the cytoplasm. Results represent the mean of three independent determinations with standard deviations up to 15% (ie, 11% ± 1.6%).

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