γ-Irradiation Induces Identical Cell Cycle Responses in Normal, Uncorrected FA(C), FAC-Corrected FA(C) (pLFACSN), and Mock-Corrected FA(C) (pLXSN) Cell Lines
| Cell Line . | %G1 . | %S . | G1/S Ratio . | %G2/M . |
|---|---|---|---|---|
| JY control | 55.9 ± 6.5 | 36.7 ± 6.9 | 1.6 ± 0.6 | 7.4 ± 2.3 |
| JY radiation | 51.2 ± 4.9 | 14.0 ± 4.9 | 4.1 ± 1.6 | 34.9 ± 8.4 |
| PD149L control | 61.9 ± 1.0 | 26.7 ± 3.5 | 1.6 ± 0.6 | 11.3 ± 2.4 |
| PD149L radiation | 58.5 ± 1.2 | 4.6 ± 4.5 | 11.3 ± 4.2 | 37.0 ± 5.0 |
| PD4L control | 58.1 ± 4.0 | 32.4 ± 5.6 | 1.9 ± 0.5 | 9.5 ± 1.7 |
| PD4L radiation | 64.2 ± 3.0 | 16.1 ± 1.8 | 4.0 ± 0.7 | 19.7 ± 2.4 |
| PD4L pLXSN control | 61.4 ± 2.2 | 29.5 ± 2.1 | 2.1 ± 0.2 | 9.1 ± 1.0 |
| PD4L pLXSN radiation | 65.6 ± 3.1 | 20.1 ± 2.7 | 3.3 ± 0.6 | 14.4 ± 3.2 |
| PD4L pLFACSN control | 63.2 ± 3.8 | 28.2 ± 1.7 | 2.3 ± 0.3 | 8.6 ± 2.5 |
| PD4L pLFACSN radiation | 61.3 ± 1.8 | 19.5 ± 1.7 | 3.2 ± 0.3 | 19.3 ± 2.8 |
| HSC536N control | 53.6 ± 2.6 | 31.0 ± 4.0 | 1.7 ± 0.2 | 15.3 ± 6.2 |
| HSC536N radiation | 50.2 ± 5.4 | 12.4 ± 2.7 | 4.2 ± 0.7 | 37.3 ± 7.0 |
| HSC536N pLXSN control | 49.3 ± 1.4 | 37.0 ± 6.0 | 1.4 ± 0.2 | 11.3 ± 8.7 |
| HSC536N pLXSN radiation | 42.7 ± 4.9 | 13.4 ± 3.0 | 3.2 ± 0.4 | 43.9 ± 7.9 |
| HSC536N pLFACSN control | 44.8 ± 7.0 | 42.2 ± 13.8 | 1.2 ± 0.5 | 13.1 ± 8.9 |
| HSC536N pLFACSN radiation | 42.7 ± 8.0 | 16.3 ± 3.2 | 2.7 ± 0.4 | 41.0 ± 10.5 |
| Cell Line . | %G1 . | %S . | G1/S Ratio . | %G2/M . |
|---|---|---|---|---|
| JY control | 55.9 ± 6.5 | 36.7 ± 6.9 | 1.6 ± 0.6 | 7.4 ± 2.3 |
| JY radiation | 51.2 ± 4.9 | 14.0 ± 4.9 | 4.1 ± 1.6 | 34.9 ± 8.4 |
| PD149L control | 61.9 ± 1.0 | 26.7 ± 3.5 | 1.6 ± 0.6 | 11.3 ± 2.4 |
| PD149L radiation | 58.5 ± 1.2 | 4.6 ± 4.5 | 11.3 ± 4.2 | 37.0 ± 5.0 |
| PD4L control | 58.1 ± 4.0 | 32.4 ± 5.6 | 1.9 ± 0.5 | 9.5 ± 1.7 |
| PD4L radiation | 64.2 ± 3.0 | 16.1 ± 1.8 | 4.0 ± 0.7 | 19.7 ± 2.4 |
| PD4L pLXSN control | 61.4 ± 2.2 | 29.5 ± 2.1 | 2.1 ± 0.2 | 9.1 ± 1.0 |
| PD4L pLXSN radiation | 65.6 ± 3.1 | 20.1 ± 2.7 | 3.3 ± 0.6 | 14.4 ± 3.2 |
| PD4L pLFACSN control | 63.2 ± 3.8 | 28.2 ± 1.7 | 2.3 ± 0.3 | 8.6 ± 2.5 |
| PD4L pLFACSN radiation | 61.3 ± 1.8 | 19.5 ± 1.7 | 3.2 ± 0.3 | 19.3 ± 2.8 |
| HSC536N control | 53.6 ± 2.6 | 31.0 ± 4.0 | 1.7 ± 0.2 | 15.3 ± 6.2 |
| HSC536N radiation | 50.2 ± 5.4 | 12.4 ± 2.7 | 4.2 ± 0.7 | 37.3 ± 7.0 |
| HSC536N pLXSN control | 49.3 ± 1.4 | 37.0 ± 6.0 | 1.4 ± 0.2 | 11.3 ± 8.7 |
| HSC536N pLXSN radiation | 42.7 ± 4.9 | 13.4 ± 3.0 | 3.2 ± 0.4 | 43.9 ± 7.9 |
| HSC536N pLFACSN control | 44.8 ± 7.0 | 42.2 ± 13.8 | 1.2 ± 0.5 | 13.1 ± 8.9 |
| HSC536N pLFACSN radiation | 42.7 ± 8.0 | 16.3 ± 3.2 | 2.7 ± 0.4 | 41.0 ± 10.5 |
Cell cycle parameters were measured in untreated cells and cells that had been irradiated 24 hours previously. Irradiated cells received a dose of 500 rads. Similar results were obtained using cells that were analyzed 6 hours after radiation. G1/S ratio = G1%/S%. Values represent mean ± 1 SD. The cumulative results of four independent experiments are shown (except for JY [n = 5] and PD149L [n = 3]).