HU Induces Identical Cell Cycle Responses in Normal and FA(C) Cells
| Cell Line . | HU Dose (mmol/L) . | %G1 . | %S . | G1/S Ratio . | %G2/M . |
|---|---|---|---|---|---|
| JY (n = 5) | 0 | 63.6 ± 7.2 | 28.6 ± 4.1 | 2.3 ± 0.7 | 7.7 ± 0.5 |
| JY (n = 5) | 5 | 87.2 ± 4.7 | 12.8 ± 4.7 | 7.8 ± 3.4 | 0.0 ± 0.0 |
| PD4L (n = 5) | 0 | 61.3 ± 2.4 | 25.5 ± 2.7 | 2.4 ± 0.3 | 13.3 ± 1.5 |
| PD4L (n = 5) | 5 | 80.5 ± 1.4 | 17.9 ± 1.6 | 4.5 ± 0.4 | 1.7 ± 1.7 |
| PD4L pLXSN (n = 3) | 0 | 62.9 ± 2.1 | 27.1 ± 2.5 | 2.3 ± 0.2 | 10.1 ± 2.7 |
| PD4L pLXSN (n = 3) | 5 | 85.4 ± 1.8 | 14.4 ± 2.0 | 6.0 ± 0.9 | 0.2 ± 0.3 |
| PD4L pLFACSN (n = 3) | 0 | 65.7 ± 3.7 | 28.4 ± 3.4 | 2.3 ± 0.4 | 5.8 ± 2.6 |
| PD4L pLFACSN (n = 3) | 5 | 81.9 ± 3.2 | 16.4 ± 2.2 | 5.1 ± 0.9 | 1.8 ± 2.2 |
| PD149L (n = 2) | 0 | 61.8 ± 1.2 | 24.4 ± 0.4 | 2.5 ± 0.1 | 13.8 ± 0.7 |
| PD149L (n = 2) | 5 | 83.4 ± 5.9 | 10.9 ± 2.7 | 8.0 ± 2.5 | 5.8 ± 3.2 |
| HSC536N (n = 4) | 0 | 62.3 ± 4.6 | 26.4 ± 4.2 | 2.4 ± 0.5 | 11.4 ± 0.6 |
| HSC536N (n = 4) | 5 | 76.4 ± 10.8 | 21.1 ± 10.5 | 4.8 ± 3.3 | 2.9 ± 1.5 |
| HSC536N pLXSN (n = 4) | 0 | 58.7 ± 4.2 | 30.3 ± 3.5 | 2.0 ± 0.3 | 11.1 ± 2.1 |
| HSC536N pLXSN (n = 4) | 5 | 74.8 ± 16.0 | 24.0 ± 14.8 | 4.8 ± 3.7 | 1.2 ± 1.7 |
| HSC536N pLFACSN (n = 3) | 0 | 55.6 ± 4.8 | 31.7 ± 3.3 | 1.8 ± 0.0 | 12.7 ± 8.1 |
| HSC536N pLFACSN (n = 3) | 5 | 73.4 ± 8.6 | 20.8 ± 11.4 | 4.3 ± 2.1 | 5.8 ± 5.8 |
| Cell Line . | HU Dose (mmol/L) . | %G1 . | %S . | G1/S Ratio . | %G2/M . |
|---|---|---|---|---|---|
| JY (n = 5) | 0 | 63.6 ± 7.2 | 28.6 ± 4.1 | 2.3 ± 0.7 | 7.7 ± 0.5 |
| JY (n = 5) | 5 | 87.2 ± 4.7 | 12.8 ± 4.7 | 7.8 ± 3.4 | 0.0 ± 0.0 |
| PD4L (n = 5) | 0 | 61.3 ± 2.4 | 25.5 ± 2.7 | 2.4 ± 0.3 | 13.3 ± 1.5 |
| PD4L (n = 5) | 5 | 80.5 ± 1.4 | 17.9 ± 1.6 | 4.5 ± 0.4 | 1.7 ± 1.7 |
| PD4L pLXSN (n = 3) | 0 | 62.9 ± 2.1 | 27.1 ± 2.5 | 2.3 ± 0.2 | 10.1 ± 2.7 |
| PD4L pLXSN (n = 3) | 5 | 85.4 ± 1.8 | 14.4 ± 2.0 | 6.0 ± 0.9 | 0.2 ± 0.3 |
| PD4L pLFACSN (n = 3) | 0 | 65.7 ± 3.7 | 28.4 ± 3.4 | 2.3 ± 0.4 | 5.8 ± 2.6 |
| PD4L pLFACSN (n = 3) | 5 | 81.9 ± 3.2 | 16.4 ± 2.2 | 5.1 ± 0.9 | 1.8 ± 2.2 |
| PD149L (n = 2) | 0 | 61.8 ± 1.2 | 24.4 ± 0.4 | 2.5 ± 0.1 | 13.8 ± 0.7 |
| PD149L (n = 2) | 5 | 83.4 ± 5.9 | 10.9 ± 2.7 | 8.0 ± 2.5 | 5.8 ± 3.2 |
| HSC536N (n = 4) | 0 | 62.3 ± 4.6 | 26.4 ± 4.2 | 2.4 ± 0.5 | 11.4 ± 0.6 |
| HSC536N (n = 4) | 5 | 76.4 ± 10.8 | 21.1 ± 10.5 | 4.8 ± 3.3 | 2.9 ± 1.5 |
| HSC536N pLXSN (n = 4) | 0 | 58.7 ± 4.2 | 30.3 ± 3.5 | 2.0 ± 0.3 | 11.1 ± 2.1 |
| HSC536N pLXSN (n = 4) | 5 | 74.8 ± 16.0 | 24.0 ± 14.8 | 4.8 ± 3.7 | 1.2 ± 1.7 |
| HSC536N pLFACSN (n = 3) | 0 | 55.6 ± 4.8 | 31.7 ± 3.3 | 1.8 ± 0.0 | 12.7 ± 8.1 |
| HSC536N pLFACSN (n = 3) | 5 | 73.4 ± 8.6 | 20.8 ± 11.4 | 4.3 ± 2.1 | 5.8 ± 5.8 |
Cells were treated with control media or media containing 5 mmol/L HU for 24 hours. Following treatment, cell cycle parameters were measured by propidium iodide staining and flow cytometry. G1/S ratio = G1%/S%. Data are represented as mean ± 1 SD. Similar results were obtained using 1 mmol/L HU (data not shown).