Treatment of FA(C) or FAC-Corrected FA(C) Cells With Equitoxic Doses of MMC Results in Similar G2/M Compartment Accumulation
| Cell Line . | MMC Dose (nmol/L) . | % Inhibition . | Delta G2/M% (24 h) . | Delta G2/M% (48 h) . | Delta S% (24 h) . | Delta S% (48 h) . |
|---|---|---|---|---|---|---|
| HSC536N | 4 | 73.3 ± 8.7 | 13.0 ± 2.4 | 7.2 ± 2.3 | −4.8 ± 4.5 | −4.9 ± 4.9 |
| HSC536N pLFACSN | 100 | 73.5 ± 4.1 | 11.9 ± 2.5 | 13.5 ± 2.5 | 3.7 ± 4.7 | 4.1 ± 3.7 |
| HSC536N | 8 | 89.4 ± 5.7 | 18.3 ± 1.5 | 16.9 ± 3.3 | −9.5 ± 3.6 | −9.8 ± 6.5 |
| HSC536N pLFACSN | 125 | 91.9 ± 1.7 | 13.7 ± 3.5 | 16.6 ± 1.8 | 4.3 ± 6.5 | −11.3 ± 4.1 |
| Cell Line . | MMC Dose (nmol/L) . | % Inhibition . | Delta G2/M% (24 h) . | Delta G2/M% (48 h) . | Delta S% (24 h) . | Delta S% (48 h) . |
|---|---|---|---|---|---|---|
| HSC536N | 4 | 73.3 ± 8.7 | 13.0 ± 2.4 | 7.2 ± 2.3 | −4.8 ± 4.5 | −4.9 ± 4.9 |
| HSC536N pLFACSN | 100 | 73.5 ± 4.1 | 11.9 ± 2.5 | 13.5 ± 2.5 | 3.7 ± 4.7 | 4.1 ± 3.7 |
| HSC536N | 8 | 89.4 ± 5.7 | 18.3 ± 1.5 | 16.9 ± 3.3 | −9.5 ± 3.6 | −9.8 ± 6.5 |
| HSC536N pLFACSN | 125 | 91.9 ± 1.7 | 13.7 ± 3.5 | 16.6 ± 1.8 | 4.3 ± 6.5 | −11.3 ± 4.1 |
Cells were treated with control media or media containing MMC, and cell cycle parameters were measured at 24 and 48 hours and cellular proliferation at 120 hours. Percent inhibition = (1 − MMC proliferation/control proliferation) × 100 ± 1 SD. Delta G2/M% = G2/M% (MMC) − G2/M% (control) and is expressed as the difference ± 95% confidence intervals. Delta S% = S% (MMC) − S% (control) and is expressed as the difference ± 95% confidence intervals. The results of a representative experiment are shown.