Normal and FA(C) Cells With Similar Amounts of MMC-Induced Cytogenetic Abnormalities Have Similar G2/M Accumulation
| Cell Line . | MMC Dose (ng/mL) . | Delta G2/M% . | Delta S% . | DNA Damage (breaks/cell) . | DNA Damage (% radials) . |
|---|---|---|---|---|---|
| JY | 200 | 25.6 ± 0.9 | 2.80 ± 1.8 | 0.89 | 24 |
| PD4L | 40 | 28.4 ± 1.1 | −6.10 ± 1.1 | 1.29 | 44 |
| JY | 200 | 20.0 ± 1.0 | 8.4 ± 2.0 | 1.38 | 36 |
| JY | 300 | 28.0 ± 1.2 | 8.9 ± 2.3 | 2.19 | 58 |
| PD149L | 40 | 40.1 ± 1.1 | −13.1 ± 1.6 | 1.86 | 42 |
| PD4L | 20 | 14.6 ± 1.2 | −9.1 ± 2.1 | 1.73 | 34 |
| PD4L | 40 | 19.4 ± 1.6 | −8.0 ± 2.6 | 2.89 | 46 |
| PD4L | 10 | 20.8 ± 1.9 | −11.6 ± 3.1 | 1.22 | 10 |
| PD4L | 40 | 30.2 ± 2.2 | −11.7 ± 3.3 | 3.14 | 30 |
| PD4L pLXSN | 10 | 14.3 ± 1.6 | −8.8 ± 3.2 | 1.07 | 10 |
| PD4L pLXSN | 40 | 21.3 ± 2.3 | −16.7 ± 2.7 | 2.41 | 32 |
| PD4L pLFACSN | 200 | 21.6 ± 1.7 | 8.6 ± 3.1 | 0.91 | 14 |
| PD4L pLFACSN | 400 | 30.4 ± 2.2 | 10.8 ± 4.0 | 1.85 | 34 |
| Cell Line . | MMC Dose (ng/mL) . | Delta G2/M% . | Delta S% . | DNA Damage (breaks/cell) . | DNA Damage (% radials) . |
|---|---|---|---|---|---|
| JY | 200 | 25.6 ± 0.9 | 2.80 ± 1.8 | 0.89 | 24 |
| PD4L | 40 | 28.4 ± 1.1 | −6.10 ± 1.1 | 1.29 | 44 |
| JY | 200 | 20.0 ± 1.0 | 8.4 ± 2.0 | 1.38 | 36 |
| JY | 300 | 28.0 ± 1.2 | 8.9 ± 2.3 | 2.19 | 58 |
| PD149L | 40 | 40.1 ± 1.1 | −13.1 ± 1.6 | 1.86 | 42 |
| PD4L | 20 | 14.6 ± 1.2 | −9.1 ± 2.1 | 1.73 | 34 |
| PD4L | 40 | 19.4 ± 1.6 | −8.0 ± 2.6 | 2.89 | 46 |
| PD4L | 10 | 20.8 ± 1.9 | −11.6 ± 3.1 | 1.22 | 10 |
| PD4L | 40 | 30.2 ± 2.2 | −11.7 ± 3.3 | 3.14 | 30 |
| PD4L pLXSN | 10 | 14.3 ± 1.6 | −8.8 ± 3.2 | 1.07 | 10 |
| PD4L pLXSN | 40 | 21.3 ± 2.3 | −16.7 ± 2.7 | 2.41 | 32 |
| PD4L pLFACSN | 200 | 21.6 ± 1.7 | 8.6 ± 3.1 | 0.91 | 14 |
| PD4L pLFACSN | 400 | 30.4 ± 2.2 | 10.8 ± 4.0 | 1.85 | 34 |
Cells were incubated with various doses of MMC for 48 hours. After treatment the cells were harvested and the samples divided for simultaneous cytogenetic and cell cycle analysis. The double lines divide independent experiments. Delta G2/M% = G2/M% (MMC) − G2/M% (control) and is expressed as the mean difference ± 95% confidence intervals. Delta S% = S% (MMC) − S% (control) and is expressed as the mean difference ± 95% confidence intervals. DNA breaks/cell = average number of DNA breaks per cell (for purposes of computation cells with radials were not included).