FAC Gene Replacement Normalizes the Cell Cycle Response of FA(C) Lymphoblasts to Low-Dose MMC
| Cell Line . | MMC (ng/mL) . | Delta G2/M% (mean ± SD) . |
|---|---|---|
| JY | 30 | 1.9 ± 0.7 (n = 3) |
| HSC536N | 30 | 18.3 ± 6.1 (n = 3) |
| HSC536N pLFACSN | 30 | 0.8 ± 0.8 (n = 4) |
| HSC536N pLXSN | 30 | 26.9 ± 0.3 (n = 2) |
| JY | 40 | 2.8 ± 2.5 (n = 4) |
| PD4L | 40 | 11.4 ± 1.5 (n = 3) |
| PD4L pLFACSN | 40 | 0.8 ± 0.1 (n = 2) |
| PD4L pLXSN | 40 | 7.3 ± 0.1 (n = 2) |
| Cell Line . | MMC (ng/mL) . | Delta G2/M% (mean ± SD) . |
|---|---|---|
| JY | 30 | 1.9 ± 0.7 (n = 3) |
| HSC536N | 30 | 18.3 ± 6.1 (n = 3) |
| HSC536N pLFACSN | 30 | 0.8 ± 0.8 (n = 4) |
| HSC536N pLXSN | 30 | 26.9 ± 0.3 (n = 2) |
| JY | 40 | 2.8 ± 2.5 (n = 4) |
| PD4L | 40 | 11.4 ± 1.5 (n = 3) |
| PD4L pLFACSN | 40 | 0.8 ± 0.1 (n = 2) |
| PD4L pLXSN | 40 | 7.3 ± 0.1 (n = 2) |
Lymphoblast cells lines were treated with MMC for 2 hours, washed extensively, and cultured in complete medium. After 24 hours of culture cells were harvested and cell cycle parameters measured by PI staining of DNA content followed by flow cytometry. Delta G2/M% = G2/M% (MMC) − G2/M% (control). Cumulative results are shown as mean ± 1 SD.