Inhibitory Effect of Anti-α2β1 MoAbs on Platelet Adhesion to Monomeric and Fibrillar Collagen Type I and III Under Flow Conditions
| Surface . | Control . | +MoAb 176D7 . | +MoAb 6F1 . |
|---|---|---|---|
| Collagen I, M | 17.7 ± 1.3 (n = 20) | 1.5 ± 0.3 (n = 12) | 8.3 ± 0.7 (n = 8) |
| Collagen I, F | 20.7 ± 2.1 (n = 18) | 1.7 ± 0.4 (n = 12) | 6.4 ± 1.1 (n = 9) |
| Collagen III, M | 15.4 ± 0.9 (n = 19) | 4.6 ± 0.7 (n = 9) | 2.9 ± 0.3 (n = 9) |
| Collagen III, F | 17.9 ± 1.0 (n = 15) | 4.5 ± 1.2 (n = 6) | 6.0 ± 0.4 (n = 9) |
| Surface . | Control . | +MoAb 176D7 . | +MoAb 6F1 . |
|---|---|---|---|
| Collagen I, M | 17.7 ± 1.3 (n = 20) | 1.5 ± 0.3 (n = 12) | 8.3 ± 0.7 (n = 8) |
| Collagen I, F | 20.7 ± 2.1 (n = 18) | 1.7 ± 0.4 (n = 12) | 6.4 ± 1.1 (n = 9) |
| Collagen III, M | 15.4 ± 0.9 (n = 19) | 4.6 ± 0.7 (n = 9) | 2.9 ± 0.3 (n = 9) |
| Collagen III, F | 17.9 ± 1.0 (n = 15) | 4.5 ± 1.2 (n = 6) | 6.0 ± 0.4 (n = 9) |
Data are expressed as the percentage of surface covered with platelets. MoAb 176D7 and MoAb 6F1, directed against the α2-subunit of the integrin α2β1, were both added at a concentration of 20 μg/mL whole blood. The perfusion experiments were performed at 300 s−1. Values are the mean ± SEM, obtained in at least three independent experiments (n = number of evaluated coverslips).
Abbreviations: M, monomeric; F, fibrillar.