Inhibitory Effect of Anti-GPIb MoAb Alone or in Combination With Anti-α2β1 MoAb on Platelet Adhesion to Monomeric Collagen Type I and III Under Flow Conditions
| Surface . | Control . | +MoAb AK-2 . | +MoAb AK-2 +MoAb 176D7 . |
|---|---|---|---|
| Collagen I | 25.4 ± 3.1 (n = 8) | 12.2 ± 0.9 (n = 9) | 4.9 ± 0.8 (n = 9) |
| Collagen III | 19.3 ± 3.1 (n = 9) | 7.2 ± 0.8 (n = 8) | 2.2 ± 0.2 (n = 8) |
| Surface . | Control . | +MoAb AK-2 . | +MoAb AK-2 +MoAb 176D7 . |
|---|---|---|---|
| Collagen I | 25.4 ± 3.1 (n = 8) | 12.2 ± 0.9 (n = 9) | 4.9 ± 0.8 (n = 9) |
| Collagen III | 19.3 ± 3.1 (n = 9) | 7.2 ± 0.8 (n = 8) | 2.2 ± 0.2 (n = 8) |
Data are expressed as the percentage of surface covered with platelets. MoAb AK-2 against GPIb was added in a 1:1,000 dilution in whole blood. MoAb 176D7, directed against the α2-subunit of the integrin α2β1, was added at a concentration of 20 μg/mL whole blood. The perfusion experiments were performed at 300 s−1. Values are the mean ± SEM, obtained in at least three independent experiments (n = number of evaluated coverslips).