Inhibition of Platelet Adhesion to Human Collagen Type I and III by CRPs in the Presence of dRGDW Under Flow Conditions
| . | Collagen I . | Collagen III . |
|---|---|---|
| Control | 31.6 ± 1.0 (n = 43) | 41.4 ± 0.8 (n = 37) |
| +CRP 1 | 21.2 ± 1.2 (n = 16) | 24.1 ± 1.0 (n = 17) |
| +CRP 1-XL | 14.8 ± 0.8 (n = 7) | 20.0 ± 0.8 (n = 8) |
| +CRP 2 | 28.9 ± 1.7 (n = 10) | 27.5 ± 1.3 (n = 3) |
| +CRP 2-XL | 13.6 ± 0.7 (n = 10) | 13.1 ± 1.0 (n = 3) |
| . | Collagen I . | Collagen III . |
|---|---|---|
| Control | 31.6 ± 1.0 (n = 43) | 41.4 ± 0.8 (n = 37) |
| +CRP 1 | 21.2 ± 1.2 (n = 16) | 24.1 ± 1.0 (n = 17) |
| +CRP 1-XL | 14.8 ± 0.8 (n = 7) | 20.0 ± 0.8 (n = 8) |
| +CRP 2 | 28.9 ± 1.7 (n = 10) | 27.5 ± 1.3 (n = 3) |
| +CRP 2-XL | 13.6 ± 0.7 (n = 10) | 13.1 ± 1.0 (n = 3) |
Data are expressed as the percentage of surface covered with platelets. The control perfusions were performed with dRGDW (200 μmol/L) alone present. The other perfusions were performed with both dRGDW and CRPs present. CRP 1 and 2 (free peptide or cross-linked) were added at a concentration of 100 μg/mL in whole blood. Collagens were human placental monomeric collagens. The perfusion experiments were performed at 300 s−1. Values are the mean ± SEM (n = number of evaluated coverslips) and were obtained in at least two independent experiments.